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Displaying 1126 - 1150 of 1261

Challenges in Capturing Oxygenase Activity In Vitro

November 1, 1999
Author(s)
V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J. Holden
Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large class of

Heteronuclear NMR and Crystallographic Studies of Wild-Type and H187Q Escherichia Coli Uracil DNA Glycosylase: Electrophilic Catalysis of Uracil Expulsion by a Neutral Histidine 187

September 14, 1999
Author(s)
A C. Drohat, G Xiao, M Tordova, J. Jagadeesh, K Pankiewicz, K A. Watanbe, G L. Gilliland, J T. Stivers
The nature of the putative general acid, His187, in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil

Detection of p53 Gene Mutation: Analysis by Single-Strand Conformation Polymorphism and Cleavase Fragment Length Polymorphism

June 1, 1999
Author(s)
C D. O'Connell, Donald H. Atha, M C. Oldenburg, J Tian, M Siebert, D Handrow, K Grooms, L Heisler, M de Arruda
We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clones were

Dynamic Partitioning of Neutral Polymers into a Single Ion Channel

June 1, 1999
Author(s)
S. M. Bezrukov, John J. Kasianowicz
Polymers partitioning into highly confined spaces can be studied using single nanometer-scale pores formed by protein ion channels. The ionic conductance of a channel depends on the state of occupancy of the pore by polymer and serves as a measure of

Electronic Structure and Spectra of Oxovanadium Silicate

January 11, 1999
Author(s)
Morris Krauss
The electronic structure and spectroscopy of the oxovanadium catalytic center on a silica surface is modeled with the molecule OV(OSIH 3)3. Excited electronic states are examined at optimized geometries for the ground singlet and triplet states. For both
Displaying 1126 - 1150 of 1261
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