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Displaying 1101 - 1125 of 1612

Lambda exonuclease digestion of CGG trinucleotide repeats

June 7, 2009
Author(s)
Richard Conroy, Alan Koretsky, John M. Moreland
Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of diVerent substrates, including one with an elongated FMR1 gene containing 120 CGG

T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements

April 7, 2009
Author(s)
Jason G. Kralj, Audrey Player, Hope Sedrick, Matt S. Munson, David Petersen, Samuel P. Forry, Paul Meltzer, Ernest Kawasaki, Laurie E. Locascio
We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microarray

Polymerization Stress Development in Dental Composites: Effect of Cavity Design Factor

March 13, 2009
Author(s)
Joseph M. Antonucci, Anthony A. Giuseppetti, Justin N. O'Donnell, Gary E. Schumacher, Drago Skrtic
Objective: To assess the effect of the cavity design factor (C-factor) on polymerization stress development (PSD) in resin composites. Methods: An experimental resin (BT resin) was prepared, which contained 2,2-bis[p-(2 hydroxy-3 -methacryloxypropoxy

Cell volume distributions reveal cell growth rates and division times

March 7, 2009
Author(s)
Michael W. Halter, John T. Elliott, Joseph B. Hubbard, Alessandro Tona, Anne L. Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable rates

Surface plasmon resonance imaging of cells and surface-associated fibronectin

February 26, 2009
Author(s)
Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant
Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for

Equilibrium Electrostatics of Responsive Polyelectrolyte Monolayers

January 14, 2009
Author(s)
Rebecca A. Zangmeister, Rastislav Levicky, Kang Wang
The physical behavior of polyelectrolytes at solid-liquid interfaces presents challenges both in measurement and in interpretation. An informative, yet often overlooked, property that characterizes the equilibrium organization of these systems is their

Robotic Patient Lift and Transfer

December 31, 2008
Author(s)
Roger V. Bostelman, James S. Albus
This book chapter provides background on wheelchairs, patient lift devices and the combination of mobile patient lift and transfer devices and research. The NIST Home Lift, Position and Rehabilitation (HLPR) Chair is detailed explaining the system, its use

The Role for Engineers in Health Care Information Systems

December 30, 2008
Author(s)
Ram D. Sriram, Steven J. Fenves
Health care informatics deals with all the processes or "software" of the health care enterprise: modeling, design, simulation and implementation of health care information systems, support and control of manufacturing and its associated supply chains, and
Displaying 1101 - 1125 of 1612
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