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Lambda exonuclease digestion of CGG trinucleotide repeats

Published

Author(s)

Richard Conroy, Alan Koretsky, John M. Moreland

Abstract

Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of diVerent substrates, including one with an elongated FMR1 gene containing 120 CGG repeats, was measured using absorption and Xuorescence spectroscopy. By use of magnetic tweezers sequence-dependent digestion rates and pausing was measured for individual lambda exonucleases. Within the triplet repeats a lower average and narrower distribution of rates and a higher frequency of pausing was observed.
Citation
European Biophysics Journal With Biophysics Letters
Volume
39
Issue
2

Keywords

Biophysics, Single molecule, Magnetic tweezers, Lambda exonuclease, Fragile X syndrome

Citation

Conroy, R. , koretsky, A. and Moreland, J. (2009), Lambda exonuclease digestion of CGG trinucleotide repeats, European Biophysics Journal With Biophysics Letters, [online], https://doi.org/10.1007/s00249-009-0502-5 (Accessed April 25, 2024)
Created June 6, 2009, Updated October 12, 2021