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A Uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

Published

Author(s)

David Serrell, Jera Law, Andrew Slifka, Roop L. Mahajan, Dudley S. Finch

Abstract

Mechanical forces play a key role in many cellular processes such as apoptosis and differentiation. A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied strain. The device works in a similar fashion to a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless AFM cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged using fluorescence and phase contrast techniques.
Citation
Biomedical Microdevices
Volume
6

Keywords

bioMEMS, cell mechanics, fibroblast

Citation

Serrell, D. , Law, J. , Slifka, A. , Mahajan, R. and Finch, D. (2008), A Uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements, Biomedical Microdevices, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=50612 (Accessed April 14, 2024)
Created November 30, 2008, Updated October 12, 2021