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Displaying 951 - 975 of 1261

Data Standards for Proteomics: Mitochondrial Two-Dimensional Polyacrylamide Gel Electrophoresis Data as a Model System

May 1, 2004
Author(s)
Veerasamy Ravichandran, G B. Vasquez, S Srivastava, M Verma, E Petricoin, Joshua Lubell, Ram D. Sriram, Peter E. Barker, G L. Gilliland
The advent of human proteomics as a major discipline has led to a reexamination of the need for consensus and a nationally sanctioned set of proteomics technology standards. Such standards for databases and data reporting may be applied to Two-Dimensional

Structure and Reaction in the Active Site of Mammalian Adenylyl Cyclase

April 8, 2004
Author(s)
Y. S. Lee, Morris Krauss
The reaction path for the catalytic conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP) by the enzyme mammalian adenylyl cyclase has been calculated theoretically using the Hartree-Fock method. The crystal structure of a

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Crystal Structure of the Complex Between Thrombin and the Central "E" Region of Fibrin

March 2, 2004
Author(s)
I Pechik, J Madrazo, M W. Mosesson, I Hernandez, G L. Gilliland, L Medved
Non-substrate interactions of thrombin with fibrin play an important role in modulating its procoagulant activity. To establish the structural basis for these interactions, we crystallized PPACK-inhibited thrombin in complex with a fragment, Eht

Dynamics of Proton Transfer in Bacteriorhodopsin

February 25, 2004
Author(s)
Y. S. Lee, Morris Krauss
Proton transfer from the cyctoplasm to the extracellular side is initiated from protonated asp96 in the cytoplasmic region towards the deprotonated Schiff base. This occurs in the transition from the late M photocycle state to the N state. A quantum

Molecular Biomarkers Used to Detect Cellular/Genetic Damage in Tissue-Engineered Skin

January 1, 2004
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, H Rodriguez
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, Tissue engineered skin (TestSkin II) was obtained, separated into its two cellular layers

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

Electrostatic Influence on Ion Transport through the alpha HL Channel

October 1, 2003
Author(s)
M Misakian, John J. Kasianowicz
The current-voltage relationship of a single Staphylococcus aureus α-hemolysin(αHL) channel is nonlinear, rectifying, and depends on the bulk pH and the ionic strength. The data are described qualitatively by a simple one-dimensional Nernst-Planck analysis
Displaying 951 - 975 of 1261
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