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Physics

Biological Physics

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Displaying 176 - 200 of 378

Algorithm for rapid determination of optical scattering parameters

October 18, 2017
Author(s)
Zachary H. Levine, Adam L. Pintar, Richelle H. Streater, Anne-Michelle R. Lieberson, Catherine C. Cooksey, Paul Lemaillet
Preliminary experiments at the NIST Spectral Tri-function Automated Reference Reflectometer (STARR) facility have been conducted with the goal of providing the diffuse optical properties of a solid reference standard with optical properties similar to

Knot Energy, Complexity, and Mobility of Knotted Polymers

October 17, 2017
Author(s)
Luis F. Vargas Lara, Ahmed M. Hassan, Marc A. Mansfield, Jack F. Douglas
The Newtonian capacity C of an object is defined by the equilibrium energy, EN =1/C, of a charge distribution on a conductive object and provides a classical measure of object shape. It is well known, for example, that C is minimized for a sphere for all

Report for Dedicated JPSS VIIRS Ocean Color Calibration/Validation Cruise October 2016

October 2, 2017
Author(s)
Michael Ondrusek, Veronica P. Lance, Menghua Wang, Charles Kovach, Wesley Goode, Alex Gilerson, Sam Ahmed, Ahmed El-Habashi, Robert Foster, Matteo Ottaviani, Joaquim I. Goes, Helga de Rosario Gomes, Kali McKee, Joo Won Kang, Chuanmin Hu, Jennifer Cannizzaro, Shaojie Sun, David English, B. Carol Johnson, Zhongping Lee, Junfang Lin, Nicholas Tufillaro, Ivan Lalovic, Jasmine Nahorniak, Curtiss Davis, Mike Twardowski, Nicole Stockley, Kenneth Voss
The overall aim of the annual National Oceanic and Atmospheric Administration (NOAA) dedicated Joint Polar Satellite System (JPSS) Visible Infrared Imaging Radiometer Suite (VIIRS) ocean color calibration/validation (cal/val) cruises is to support

Consistent View of Polypeptide Chain Expansion in Chemical Denaturants from Multiple Experimental Methods

September 13, 2017
Author(s)
Alessandro Borgia, Wenwei Zheng, Karin Buholzer, Madeleine Borgia, Anja Schuler, Hagen Hoffman, Andrea Sorrano, Daniel Nettels, Klaus Gast, Alexander Grishaev, Robert Best, Benjamin Schuler
There has been a long-standing controversy regarding the effect of chemical denaturants on the dimensions of unfolded and intrinsically disordered proteins: A wide range of experimental techniques suggest that polypeptide chains expand with increasing

Non-Uniqueness in Dual-Energy CT with Beam Hardening

September 13, 2017
Author(s)
Zachary H. Levine
Purpose: The goal is to determine whether dual-energy computed tomography (CT) leads to a unique reconstruction into two basis materials. Methods and Materials: The beam hardening equation is simplified to the single voxel case. The simplified equation is

Single Molecule Nanopore Spectrometry for Peptide Detection

August 15, 2017
Author(s)
Amy Chavis, Joseph Robertson, Joseph E. Reiner, Kyle Brady, Grrace Hatmaker, Nuwan Kothalawala
Sensing and characterization of water-soluble peptides is of critical importance in a wide variety of bioapplications. Single molecule nanopore spectrometry (SMNS) is based on the idea that one can use biological protein nanopores to resolve different

Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity

June 2, 2017
Author(s)
Prakash Kulkarni, Mohit Jolly, Dongya Jia, Steven Mooney, Ajay Bhargava, Luciane Kagohara, Yihong Chen, Pengyu Hao, Yanan He, Roberft Veltri, Alexander Grishaev, Keith Weninger, Herbert Levine, John Orban
Intrinsically disordered proteins (IDPs) that lack a unique 3D structure and comprise a large fraction of the human proteome play important roles in numerous cellular functions. Prostate- Associated Gene 4 (PAGE4) is an IDP that acts as a potentiator of

The 2016 edition of the Art in Science of MicroTAS

April 21, 2017
Author(s)
Darwin R. Reyes-Hernandez
Dublin, Ireland beheld The 20th International Conference of Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) from October 23 to 27, 2016. The conference not only included a great technical program, but also outreach activities, an

Broadband spectral measurements of diffuse optical properties by an integrating sphere instrument at the National Institute of Standards and Technology

March 30, 2017
Author(s)
Paul Lemaillet, Jeeseong Hwang, Heidrun Wabnitz, Dirk Grosenick, Lin Yang, David W. Allen
Diffuse materials that approximate the optical properties of human tissue are commonly used as phantoms. In order to use the phantoms in a manner that provides consistent results relative to independent measurements, the optical properties need to be tied

Label-free hyperspectral dark-field microscopy towards quantitative scatter imaging

March 27, 2017
Author(s)
Philip Cheney, David McClatchy III, Stephen Kanick, Paul Lemaillet, David W. Allen, Daniel Samarov, Brian Pogue, Jeeseong C. Hwang
A hyperspectral dark-field microscopy technique has been developed for imaging spatially distributed diffuse reflectance spectra from light-scattering samples. In this report, quantitative scatter spectroscopy was demonstrated with a uniform scattering

Polydimethylsiloxane tissue-mimicking phantoms for quantitative optical medical imaging standards

March 24, 2017
Author(s)
Jeeseong Hwang, Hyun J. Kim, Paul Lemaillet, Dirk Grosenick, Thomas Gladytz, David McClatchy III, Kimberly A. Briggman, Brian Pogue
We report on a procedure to build and characterize solid tissue-mimicking phantoms of polydimethylsiloxane (PDMS) polymers. Controlled inclusion of light scattering titanium dioxide (TiO2) nanoparticles enables the creation of phantoms having tunable light

Direct measurement of Vorticella contraction force by micropipette deflection

February 17, 2017
Author(s)
Danielle C. France, Jonathan Tejada, Paul Matsudaira
The ciliated protozoan Vorticella convallaria is noted for its exceptionally fast ATP-independent cellular contraction, but direct measurements of contractile force have proven difficult given the length scale, speed, and forces involved. We used high
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