Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Determination of Lipid Phase Transition Temperatures in Hybrid Bilayer Membranes



Neil A. Anderson, Lee J. Richter, John C. Stephenson, Kimberly A. Briggman


The main gel to liquid crystal (LC) phase transition temperature, Tm, of the lipid monolayers in hybrid bilayer membranes (HBMs) was investigated using vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to probe order/disorder transitions was used to determine Tm for a series of saturated phosphatidylcholines on octadecanethiolate self assembled monolayers (SAMs). The values obtained for Tm average 10 degrees C higher for the lipids in HBMs than for the corresponding lipids in vesicles in solution.
Journal of the American Chemical Society


hybrid bilayer membranes, phase transition temperature., SFG, supported bilayer membrane mimics, vibrational sum frequency spectroscopy


Anderson, N. , Richter, L. , Stephenson, J. and Briggman, K. (2006), Determination of Lipid Phase Transition Temperatures in Hybrid Bilayer Membranes, Journal of the American Chemical Society, [online], (Accessed May 27, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created September 9, 2006, Updated January 27, 2020