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Determination of Lipid Phase Transition Temperatures in Hybrid Bilayer Membranes

Published

Author(s)

Neil A. Anderson, Lee J. Richter, John C. Stephenson, Kimberly A. Briggman

Abstract

The main gel to liquid crystal (LC) phase transition temperature, Tm, of the lipid monolayers in hybrid bilayer membranes (HBMs) was investigated using vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to probe order/disorder transitions was used to determine Tm for a series of saturated phosphatidylcholines on octadecanethiolate self assembled monolayers (SAMs). The values obtained for Tm average 10 degrees C higher for the lipids in HBMs than for the corresponding lipids in vesicles in solution.
Citation
Journal of the American Chemical Society
Volume
22
Issue
20

Keywords

hybrid bilayer membranes, phase transition temperature., SFG, supported bilayer membrane mimics, vibrational sum frequency spectroscopy

Citation

Anderson, N. , Richter, L. , Stephenson, J. and Briggman, K. (2006), Determination of Lipid Phase Transition Temperatures in Hybrid Bilayer Membranes, Journal of the American Chemical Society, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=841012 (Accessed December 5, 2024)

Issues

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Created September 9, 2006, Updated January 27, 2020