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Displaying 176 - 200 of 848

Identification and quantification of DNA repair protein poly(ADP ribose) polymerase 1 (PARP1) in human tissues and cultured cells by liquid chromatography/isotope-dilution tandem mass spectrometry

March 1, 2019
Author(s)
Erdem Coskun, Gamze Tuna, Pawel Jaruga, Alessandro Tona, Onur Erdem, M Miral Dizdar
Poly(ADP ribose) polymerase 1 (PARP1) is a multifunctional DNA repair protein of the base excision repair pathway and plays a major role in the repair of DNA strand breaks and in replication and transcriptional regulation among other functions. Mounting

NIST Micronutrients Measurement Quality Assurance Program: 1984-1985 Comparability Studies Results for Round Robins I, II, III and V Fat Soluble Vitamins and Carotenoids in Human Serum

February 8, 2019
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, William A. MacCrehan, Robert Paule, Reenie Parris, Willie May, Robert Schaffer
From 1984 to 2017, the National Institute of Standards and Technology (NIST) and its precursor the National Burearu of Standards (NBS) coordinated what became the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure

CrowdVariant: a crowdsourcing approach to curate copy number variants

January 6, 2019
Author(s)
Justin M. Zook, Marc L. Salit, Peyton Greenside, Ryan Poplin, Mark DePristo, Madeleine Cule
Copy number variants (CNVs) are an important type of genetic variation and play a causal role in many diseases. However, they are also notoriously difficult to identify accurately from next-generation sequencing (NGS) data. For larger CNVs, genotyping

Effects of Consumer Use Practices on Nanosilver Release from Commercially Available Food Contact Materials

October 23, 2018
Author(s)
Susana Addo Ntim, Samuel R. Norris, David Goodwin, Jens Breffke, Keana Scott, Li Piin Sung, Treye Thomas, Gregory O. Noonan
Migration evaluation involving nano-enabled food contact materials (FCMs) mostly focus on potential nanoparticle release from new unused products. This may not represent consumer use practices encountered by the FCMs in their lifecycle. In order to

Innovative Approaches to Combat Healthcare-Associated Infections Using Efficacy Standards Developed Through Industry and Federal Collaboration

October 5, 2018
Author(s)
Dianne L. Poster, Carl C. Miller, Yaw S. Obeng, Michael T. Postek, Troy E. Cowan, Richard A. Martinello
Nation-wide, healthcare-associated infections (HAIs) infect one in every 25 hospital patients, account for more than 100,000 deaths and increase medical costs by around $96-147B, each year. Ultraviolet-C (UV-C) antimicrobial devices are shown to reduce the

Combinatorial Cassettes for Higher-Throughput Screening of Osteogenesis

October 4, 2018
Author(s)
Carl G. Simon Jr., Subhadip Bodhak, Hariharan K. Iyer, Luis Fernandez de Castro Diaz, Pam Robey, Azusa Maeda, Sergei Kuznetsov
Animal models are the most biologically relevant method for measuring the osteogenic capability of bone graft formulations. However, animal experiments are slow, tedious and the results can be highly variable, even when conducted within the same lab. In

Simmering Sauces! Elevated Formaldehyde Concentrations from Gas Stove Burners

July 22, 2018
Author(s)
Dustin G. Poppendieck, Mengyan Gong
Formaldehyde is listed as a human carcinogen, and can be formed as a primary by-product during combustion processes. Past studies have not detected elevated formaldehyde concentrations from gas stoves, perhaps due to the time averaging nature of previous

An update on dose calibrator settings for nuclides used in nuclear medicine

June 1, 2018
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna
Most clinical measurements of radioactivity, whether for therapeutic or imaging nuclides, rely on commercial reentrant ionization chambers (“dose calibrators”). The National Institute of Standards and Technology (NIST) maintains a battery of representative

Standardization of 64Cu activity

May 25, 2018
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna, Ryan P. Fitzgerald, Leticia S. Pibida, Brian E. Zimmerman
The complex decay scheme that makes 64Cu promising as both an imaging and therapeutic agent in medicine also makes the absolute measurement of its activity challenging. The National Institute of Standards and Technology (NIST) has completed a primary

NIST Micronutrients Measurement Quality Assurance Program: Spring and Fall 1987 Comparability Studies-Results for Round Robins IX and XI Fat-Soluble Vitamins and Carotenoids in Human Serum

May 21, 2018
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, Willie May, Emil Schonberger
From 1984 to 2017, the National Institute of Standards and Technology coordinated the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- soluble vitamins and carotenoids in human serum and plasma. This report

Opportunities and Challenges in Implementation of Multiparameter Single Cell Analysis Platforms for Clinical Translation

May 11, 2018
Author(s)
susan M. Keating, D. Lansing Taylor, Anne L. Plant, E. David Litwack, Peter Kuhn, Emily J. Greenspan, Christopher M. Hartshorn, Caroline C. Sigman, Gary J. Kelloff, Gregory Friberg, Jerry S. Lee, Keisuke Kuida
The high-content interrogation of single cells with platforms optimized for the multiparameter characterization of cells in liquid and solid biopsy samples can enable characterization of heterogeneous populations of cells ex vivo. Doing so will advance the

Oxidatively-induced DNA damage and base excision repair in euthymic patients with bipolar disorder

May 1, 2018
Author(s)
Deniz Ceylan, Gamze Tuna, Guldal Kirkali, Zeliha Tunca, Gunes Can, Hidayet E. Arat, Melis Kant, Miral M. Dizdar, Aysegul Ozerdem
Oxidatively-induced DNA damage has previously been associated with bipolar disorder. More recently, impairments in DNA repair mechanisms have also been reported. We aimed to investigate oxidatively-induced DNA lesions and expression of DNA glycosylases

Qualification of NISTmAb charge heterogeneity control assays

March 18, 2018
Author(s)
Abigail Turner, John E. Schiel
The NISTmAb is a monoclonal antibody Reference Material from the National Institute of Standards and Technology; it is a class-representative IgG1κ intended serve as a pre-competitive platform for harmonization and technology development in the

Development of an LC-MS/MS peptide mapping protocol for the NISTmAb

March 1, 2018
Author(s)
Catherine A. Mouchahoir, John E. Schiel
Peptide mapping is a component of the analytical toolbox used within the biopharmaceutical industry to aid in the identity confirmation of a protein therapeutic and to monitor degradative events such as oxidation or deamidation. These methods offer the

Standard Reference Material 2378 Fatty Acids in Frozen Human Serum. A Clinical SRM based on Endogenous Supplementation of Polyunsaturated Fatty Acids

February 13, 2018
Author(s)
Bruce A. Benner Jr., Michele M. Schantz, Johanna Camara, Katherine E. Sharpless, James H. Yen, Lorna T. Sniegoski, Carissa D. Powers, Rosemary L. Schleicher
Dietary fatty acids can be both beneficial and detrimental to human health depending on the degree and type of saturation. Healthcare providers and research scientists monitor the fatty acid content of human plasma and serum as an indicator of health

Effects of Distal Residues Mutations on the Structure, Dynamics and Catalysis of Human Monoacylglycerol Lipase

January 29, 2018
Author(s)
Sergiy Tyukhtenko, Girija Rajarshi, Ioannis Karageorgos, Nikolai Zvonok, Elyssia S. Gallagher, Hongwei Huang, Kiran Vemuri, Jeffrey W. Hudgens, Alexandros Makriyannis
An understanding of how conformational dynamics modulates function and catalysis of human monoacylglycerol lipase (hMGL), an important pharmaceutical target, can facilitate the development of novel modulatory ligands. Here, we report the discovery and

Recommendations for assessing commutability part 1: general experimental design

January 18, 2018
Author(s)
Johanna Camara, W. Greg Miller, Schimmel Heinz, Neil Greenberg, Chris Burns, Vincent Delatour, Mauro Panteghini, Hubert W. Vesper, Robert Rej, Ferruccio Ceriotti, Gerhard Schumann, Jeffrey R. Budd, Weykamp Cas, Goran Nilsson, Finlay MacKenzie, Thomas Keller
Commutability is a property of a reference material that relates to the closeness of agreement between results for a reference material and results for clinical samples when measured by two or more measurement procedures. Commutability of reference

Recommendations for assessing commutability part 2: based on the difference in bias between a reference material and clinical samples

January 18, 2018
Author(s)
Johanna Camara, Goran Nilsson, Jeffrey R. Budd, Neil Greenberg, Vincent Delatour, Gerhard Schumann, Robert Rej, Mauro Panteghini, Ferruccio Ceriotti, Schimmel Heinz, Weykamp Cas, Thomas Keller, Chris Burns, Finlay MacKenzie, W. Greg Miller
A process is described to assess the commutability of a reference material intended for use as a calibrator, trueness control or external quality assessment sample based on the difference in bias between a reference material and clinical samples measured

Recommendations for assessing commutability part 3: based on the calibration effectiveness of a reference material

January 18, 2018
Author(s)
Johanna Camara, Weykamp Cas, Goran Nilsson, Robert Rej, Finlay MacKenzie, Ferruccio Ceriotti, Neil Greenberg, Schimmel Heinz, Gerhard Schumann, Thomas Keller, Vincent Delatour, Mauro Panteghini, Chris Burns, W. Greg Miller
A process is described to assess the commutability of a reference material intended for use as a calibrator based on its ability to fulfill its intended use in a calibration traceability scheme to produce equivalent clinical sample results among different

Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

December 1, 2017
Author(s)
Darion Spigolon, David Travis Gallagher, Adrian Velazquez-Campoy, Donatella Bulone, Jatin Narang, Pier San Biagio, Francesco Cappello, Alberto J. Macario, Everly Conway de Macario, Frank Robb
The human chaperonin complex is a 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy

Tissue Inhibitor of Metalloprotease-2: bioprocess development, physicochemical, biochemical and biological characterization of highly expressed recombinant protein

December 1, 2017
Author(s)
Ananda Chowdhury, Robert G. Brinson, Beiyang Wei, William G. Stetler-Stevenson
Tissue Inhibitor of Metalloprotease -2 (TIMP-2) is a secreted, 21 kDa, multifunctional protein. It was first described as an endogenous inhibitor of matrix metalloproteases (MMPs) that prevents protease-mediated breakdown of the extracellular matrix often
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