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Forensic Science

Forensic genetics

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Displaying 1 - 25 of 252

Development of Publicly Available Forensic DNA Sequence Mixture Data

March 12, 2025
Author(s)
Erica Romsos, Kevin Kiesler, Carolyn Steffen, Lisa Borsuk, Sarah Riman, Lauren Mullen, Jodi Irwin, Peter Vallone, Katherine Gettings
Background: In 2018, the Next-Generation Sequencing Committee of SWGDAM queried bioinformatic and statistical interpretation method developers regarding data needs for the development of sequence-based probabilistic genotyping software. Methods: Based on

DNA Mixture Interpretation: A NIST Scientific Foundation Review

December 18, 2024
Author(s)
John Butler, Hariharan Iyer, Richard Press, Melissa Taylor, Peter Vallone, Sheila Willis
Improvements in DNA testing methods have allowed forensic scientists to reduce the quantity of DNA required for profiling an individual. Today, DNA profiles can be generated from a few skin cells. This increased sensitivity has extended the usefulness of

Results of the 2023 Rapid DNA Multi-Laboratory Study - I-Chip

December 4, 2024
Author(s)
Erica Romsos, Lilliana Moreno, Douglas Hares
Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the

Development of a Forensic DNA Research Grade Test Material

October 16, 2024
Author(s)
Erica Romsos, Lisa Borsuk, Carolyn Steffen, Sarah Riman, Kevin Kiesler, Peter Vallone
Advancements in forensic DNA typing technology and methods have resulted in increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex

Harmonizing the forensic nomenclature for STR Loci D6S474 and DYS612

January 21, 2024
Author(s)
Martin Bodner, David Ballard, Lisa Borsuk, Jonathan King, Walther Parson, Christopher Phillips, Katherine Gettings
The autosomal short tandem repeat (STR) locus D6S474 and the Y-chromosomal STR locus DYS612 have been reported multiple ways in the forensic literature, with differences in both the bracketed repeat structures and counting of numerical length-based

STRSeq: FAQ for submitting

December 1, 2022
Author(s)
Lisa Borsuk, Peter Vallone, Katherine Gettings
The STR sequencing project was developed due to the necessity of publicly sharing sequencing information about Short Tandem Repeats (STR) associated with human identification. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) is

Sequence-based U.S. population data for 7 X STR loci

April 26, 2021
Author(s)
Lisa Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone, Katherine Gettings
The National Institute of Standards and Technology (NIST) U.S. population sample set of unrelated individuals was used to determine allele and haplotype frequencies for seven X-chromosome short tandem repeat (STR) loci in four linkage groups. DXS7132

Classification of STR allelic variation using massively parallel sequencing and assessment of flanking region power

July 12, 2020
Author(s)
Laurence Devesse, Lucinda Davenport, Lisa Borsuk, Katherine Gettings, Gabriella Mason-Buck, Peter Vallone, David Ballard
The application of massively parallel sequencing (MPS) to forensic genetics has led to improvements in multiple aspects of DNA analysis, however, additional complexities are concurrently associated with these advances. In relation to short tandem repeat

DNA mixture interpretation principles: insights from the NIST scientific foundation review

July 1, 2020
Author(s)
John Butler, Hariharan Iyer, Richard Press, Melissa Taylor, Peter Vallone, Sheila Willis
DNA mixture interpretation has become more challenging in recent years due to several factors including submission of more touch evidence samples to aid investigations and generation of more sensitive DNA test results with new STR typing kits. This

Results of the 2018 Rapid DNA Maturity Assessment

January 27, 2020
Author(s)
Erica L. Romsos, Peter Vallone
Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in the July of 2018. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the

Understanding the characteristics of sequence-based single-source DNA profiles

November 9, 2019
Author(s)
Sarah Riman, Hariharan K. Iyer, Lisa A. Borsuk, Peter M. Vallone
The sequencing of STR markers provides additional information present in the underlying sequence variation that is typically masked by traditional fragment-based genotyping. However, the interpretation of STR profiles generated by targeted sequencing
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