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Barbara C. Levin, L A. Tully, Diane K. Hancock, Frederick P. Schwarz, M S. Lee
Every human cell has from a few dozen to several thousand mitochondria each of which contain mitochondrial DNA (mtDNA). The sequence of the entire human mtDNA (16,569 base pairs) was determined and published by Anderson et al. in 1981. A mtDNA standard
This document describes the testing of dd (GNU fileutils) 4.0.36 as a disk imaging tool on a Linux platform. The Linux version used was Linux version 2.4.2-2 (Red Hat Linux 7.1 2.96-79). The test cases that were applied are described in Disk Imaging Tool
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)and ion pair-reverse phase high performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stranded
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In
Mention has already been made, in Section 5.1. Of the use of DNA-based techniques for control of microbiological DNA. In parallel to the work of ATCC the USA National Institute of Standards and Technology (NIST) developed three Standard Reference Materials
Kristy Lenz, Dawn Rabbach, Chao Liu, Quyi Xu, Ying Zeng, Becky Steffen, Douglas Storts
The VersaPlex™ 27PY System is an STR multiplex from Promega offering co-amplification of 27 loci. This six-dye multiplex was designed for casework samples and includes loci that meet database requirements for the US (CODIS), Europe (ESS) and China (Chinese
The National Institute of Standards and Technology (NIST) has compiled and maintained a Short Tandem Repeat DNA Internet Database (http://www.cstl.nist.gov/biotech/strbase/) since 1997 commonly referred to as STRBase. This database is an information
The field of human mitochondrial genetics has advanced way beyond where the Human Genome Project hopes to be by the year 2003, the projected year for completing the sequence of the entire human nuclear DNA genome. Not only has the mitochondrial DNA (mtDNA)
Kristy L. Richie, M D. Goldsborough, M M. Darfler, E A. Benzinger, M L. Lovekamp, D J. Reeder, C D. O'Connell
The Polymerase Chain Reaction (PCR) has revolutionized the analysis of DNA from a variety of sources. With its sensitivity and ability to amplify degraded DNAs and small quantities of samples, coupled with fast turn-around-time, PCR is often the analytical
Forensic restriction fragment length polymorphism analyses typically provide two band size results at each genetic locus for each sample. In collaboration with the member laboratories of the Technical Working Group for DNA Analysis Methods, we have
Margaret C. Kline, Janette W. Redman, David L. Duewer, D J. Reeder
The National Institute of Standards and Technology (NIST) has provided a number of educational interlaboratory exercises to the forensic community. The recent NIST Mixed Stain Study #2 consisted of three separate sets of materials. Set 1) three stains on
The purpose of this test protocol is to compare the performance of protective gloves for use by law enforcement, corrections, and military personnel. The scope of this test protocol is limited to the types of gloves that meet the rating levels described in
This document, Forensic Sciences: Status and Needs, is the product of a two-day meeting sponsored by OLES/NIJ/NIST on March 5-6, 1997. This meeting brought together 44 scientists and administrators with the common goal of helping the forensic science
Designing and building a forensic laboratory is a complicated undertaking. Design issues include those considerations present when designing any building, with enhanced concern and special requirements involving environmental health and safety, hazardous