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Bioscience

Proteomics

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Displaying 76 - 80 of 80

The Hybrid Search: A Mass Spectral Library Search Method for Discovery of Modifications in Proteomics

April 3, 2017
Author(s)
Meghan C. Burke, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Sanford P. Markey, Stephen E. Stein, Jenny Heidbrink Thompson, Christopher Larkin
We present a mass spectral library based method to identify tandem mass spectra of peptides that contain unanticipated modifications and amino acid variants. We describe this as a ‘hybrid’ method since it combines matching both ion m/z and mass losses. The

Measurements of translation initiation from all 64 codons in E. coli

February 21, 2017
Author(s)
Ariel H. Hecht, Jeff E. Glasgow, Paul Jaschke, Lukmaan Bawazer, Matthew S. Munson, Jennifer R. Cochran, Drew Endy, Marc L. Salit
Our understanding of translation represents a cornerstone of molecular biology and underpins our capacity to engineer living matter. For decades, the codon AUG and a few near-cognates (GUG, UUG) have been exclusively considered as “start codons” for

A new approach to assess mAb aggregation

January 1, 2001
Author(s)
Illarion Turko
A proof-of-concept for new methodology to detect and potentially quantify mAb aggregation is presented. Assay development included using an aggregated mAb as bait for screening of a phage display peptide library and identifying those peptides with random
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