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Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1)

October 26, 2017

Author(s)

Natalia Mast, Joseph Lin, Kyle Anderson, Ingemar Bjorkhem, Irina A. Pikuleva

Cytochrome P450 46A1 (CYP46A1) converts cholesterol to 24-hydroxycholesterol and thereby controls the major pathways of cholesterol removal from the brain. Cyp46a1-/- mice have a reduction in the rate of cholesterol biosynthesis in the brain and

Assessment of extracellular vesicles purity using proteomic standards

September 26, 2017

Author(s)

Tingting Wang, Kyle Anderson, Illarion Turko

The increasing interest in extracellular vesicles (EVs) research is fueled by reports indicating their unique role in intercellular communication and potential connection to the development of common human diseases. The unique role assumes unique protein

The Hybrid Search: A Mass Spectral Library Search Method for Discovery of Modifications in Proteomics

April 3, 2017

Author(s)

Meghan C. Burke, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Sanford P. Markey, Stephen E. Stein, Jenny Heidbrink Thompson, Christopher Larkin

We present a mass spectral library based method to identify tandem mass spectra of peptides that contain unanticipated modifications and amino acid variants. We describe this as a hybrid method since it combines matching both ion m/z and mass losses. The

Complementary Domain Prioritization: A Method to Improve Biologically Relevant Detection in Multi-Omic Data Sets

March 3, 2017

Author(s)

Benjamin Neely, Paul E. Anderson

As the speed and quality of different analytical platforms increase, it is more common to collect data across multiple biological domains in parallel (i.e., genomics, transcriptomics, proteomics, and metabolomics). There is a growing interest in algorithms

Measurements of translation initiation from all 64 codons in E. coli

February 21, 2017

Author(s)

Ariel H. Hecht, Jeff E. Glasgow, Paul Jaschke, Lukmaan Bawazer, Matthew S. Munson, Jennifer R. Cochran, Drew Endy, Marc L. Salit

Our understanding of translation represents a cornerstone of molecular biology and underpins our capacity to engineer living matter. For decades, the codon AUG and a few near-cognates (GUG, UUG) have been exclusively considered as start codons for

A new approach to assess mAb aggregation

January 1, 2001

Author(s)

Illarion Turko

A proof-of-concept for new methodology to detect and potentially quantify mAb aggregation is presented. Assay development included using an aggregated mAb as bait for screening of a phage display peptide library and identifying those peptides with random

Quantitative proteomic analysis of biogenesis-based classification for extracellular vesicles

January 1, 2001

Author(s)

Linwen Zhang, Jeremie Parot, Vincent Hackley, Illarion Turko

terms and is much more heterogeneous and complex. Since the cell membrane origin of EVs predetermines their biological functions, the un-derstanding of EV biogenesis is important for accurate interpretation of observed results. Combining analytical