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Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications



William Davis, Lisa Kilpatrick, Debra Ellisor, Ben Neely


The National Institute of Standards and Technology (NIST) is creating new, inexpensive, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608
Scientific Data


Reference Materials, Proteomics, LC/MS/MS


Davis, W. , Kilpatrick, L. , Ellisor, D. and Neely, B. (2019), Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications, Scientific Data, [online],, (Accessed May 30, 2024)


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Created December 17, 2019, Updated October 13, 2022