Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications

Published

Author(s)

William C. Davis, Lisa E. Kilpatrick, Debra L. Ellisor, Benjamin A. Neely

Abstract

The National Institute of Standards and Technology (NIST) is creating new, inexpensive, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608
Citation
Scientific Data

Keywords

Reference Materials, Proteomics, LC/MS/MS

Citation

Davis, W. , Kilpatrick, L. , Ellisor, D. and Neely, B. (2019), Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications, Scientific Data, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=928171 (Accessed May 18, 2022)
Created December 18, 2019, Updated January 8, 2020