Proteomics

News and Updates

NIST Releases Reference Material to Aid Gut Microbiome Research

March 17, 2025

Researchers hope this material will help usher in a new era of diagnostics and treatments involving the gut microbiome.

A close-up of a virus particle shows spikes protruding from the particle's surface and blobby sugars that cling to the spikes.

New NIST Method Can Better Identify Sneaky Sugars on Viruses’ Spiky Weapons

September 28, 2023

A better picture of the sugars can increase our understanding of how humans get infected by the virus behind COVID-19 and other pathogens.

Petri dish holds brown material with several different types of circular microbes.

Spotlight: Going With the Gut for the Future of Health

June 27, 2022

NIST researchers are developing a human gut microbiome reference material.

Projects and Programs

Trusted AI framework for a new class of Standard Reference Materials and Data with exquisitely characterized uncertainty for billions of properties

Ongoing

The measurement of diverse molecular classes in biospecimens containing millions of molecules, known as omics, is increasingly important in the life sciences and medicine, and used to find novel cancer therapeutics, discover COVID-19 infection pathways, and diagnose diseases. NIST RMs and SRDs for

Performance Metrics for Proteomics

Ongoing

Intended Impact Proteomics experiments designed to identify protein markers specific to a particular disease or disease state rely on repeatable identification of proteins within a control set. Repeatability provides statistical assurance that clear differences between two sets (e.g. sick vs

Figure contains text “Omics Data Quality” and human outline with arrows pointing from the human to the following specimen types: liver, serum, stool, plasma, urine. On either side of the human are analytical spectra and everything is surrounded by a decorative molecule structure circular border.

Development of NIST Reference Materials for Untargeted Metabolomics, Lipidomics and Proteomics

Ongoing

There is a significant need for tools to increase harmonization, comparability, and reproducibility between laboratories and/or studies, and to provide quality control of sample preparation and data analysis protocols utilized in untargeted omics analysis. Reference materials are being developed to

Human Gut Microbiome Reference Material

Ongoing

The most common measurements currently being used to describe these complex microbial communities are: NGS-based metagenomics Mass spectrometry-based metabolomics In either case, no fit-for-purpose standards exist that enable researchers to compare results generated across different laboratories and

Publications

Proteomic and Lipidomic Plasma Evaluations Reveal Biomarkers for Domoic Acid Toxicosis in California Sea Lions

November 24, 2024

Author(s)

Amie Solosky, Iliana Claudio, Kaylie Kirkwood, Jessie Chappel, Michael Janech, Alison Bland, Frances Gulland, Benjamin Neely, Erin Baker

Domoic acid is a neurotoxin secreted by the marine diatom genus Pseudo-nitzschia during toxic algal bloom events. California sea lions (Zalophus californianus)

Results from a multi-laboratory ocean metaproteomic intercomparison: effects of LC-MS acquisition and data analysis procedures

November 8, 2024

Author(s)

Mak Saito, Jaclyn Saunders, Matthew McIlvin, Erin Bertrand, John Breier, Margaret Brisbin, Sophie Colston, Jaimee Compton, Tim Griffin, Judson Harvey, Robert Hettich, Pratik Jagtap, Michael Janech, Rod Johnson, Rick Keil, Hugo Kleikamp, Dasha Leary, Scott McCain, Eli Moore, Subina Mehta, Dawn Moran, Jacqui Neibauer, Benjamin Neely, Michael Jakuba, Jim Johnson, Megan Duffy, Gerhard Herndl, Richard Giannone, Ryan Mueller, Brook Nunn, Martin Pabst, Samantha Peters, Andrew Rajczewski, Elden Rowland, Brian Searle, Tim Van Den Bossche, Gary Vora, Jacob Waldbauer, Haiyan Zheng, Zihao Zhao

Metaproteomics is an increasingly popular methodology that provides information regarding the metabolic functions of specific microbial taxa and has potential

Quality Control in the Mass Spectrometry Proteomics Core: a Practical Primer

September 11, 2024

Author(s)

Ben Neely, Yasset Perez-Riverol, Magnus Palmblad

The past decade has seen widespread advances in quality control (QC) materials and software tools focused specifically on mass spectrometry–based proteomics

Variation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins

August 9, 2024

Author(s)

Zachary Goecker, Meghan Burke Harris, Concepcion Remoroza, Yi Liu, Yuri Mirokhin, Sergey Sheetlin, Dmitrii V. Tchekhovskoi, Xiaoyu (Sara) Yang, Stephen E. Stein

This work presents a detailed determination of site-specific N-glycan distributions of the recombinant influenza glycoproteins hemagglutinin (HA) and

Awards

John Marino Honored with Hillebrand Prize

John Marino is recognized for his groundbreaking contributions to the field of biophysical characterization by high-resolution NMR and