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Bioanalytical Science Group

Providing the measurement science, standards, technology, and data required to support the nation's needs in determining the composition, structure, quantity, and function of proteins, peptides, post-translational modifications, and metabolites.

We provide the measurement science, standards, technology, and data required to support the nation's needs in determining the composition, structure, quantity, and function of proteins, peptides, post-translational modifications such as glycosylation and phosphorylation, and related biomolecules such as metabolites.  In partnership with U.S. industry, government agencies, and scientific institutions, the group performs fundamental and applied research on the characterization and quantitation of proteins, peptides, and their mixtures using techniques such as mass spectrometry, liquid chromatography, electrophoresis, and immunoassays.  The Group provides measurement science, reference materials, reference data, and technologies to support accuracy and comparability of measurements needed to advance biotechnology, biomedical and bioscience research, biomanufacturing, and health care.  Specific areas of interest include measurements and standards for clinical diagnostics and biotherapeutics.

An Extraordinary Standard: New NIST Protein Could Spur Biopharmaceutical Innovation

NIST SRM 8671
Credit: Photograph taken by Matthew DeLorme

The National Institute of Standards and Technology (NIST) has issued one of the world’s most intricate measurement standards: an exhaustively analyzed antibody protein that the biopharmaceutical industry will use to help ensure the quality of treatments across a widening range of health conditions, including cancers, autoimmune disorders and infectious diseases.  Read More

News and Updates

Projects and Programs

NIST Food Protein Allergen Program

The NIST Food Protein Allergen Program is focused on providing reference materials and methods to improve the connection between the measured protein food

Publications

SURVEY OF PEPTIDE QUANTIFICATION METHODS AND THEIR REPRODUCIBILITY COMPARISON: A CASE STUDY USING OXYTOCIN

Author(s)
Chensheng Li, Sitaram Bhavaraju, Jeremy Melanson, Andreas Blomgren, Torgny Rundlof, Eric L. Kilpatrick, Timothy Rudd, Yves Aubin, Kevin Grant, Edward Saravolac, Tursun Kerim, William Sherman, Yukari Nakagawa, Sergi Pavon, Tim Weel, Arunima Pola, Dinesh Chalasani, Stephen Walfish, Fouad Atouf
USP’s peptide reference standards content is typically determined using an HPLC assay against an external standard for which the purity was determined by a mass

Harmonization of LC-MS/MS protein assays

Author(s)
Alan L. Rockwood, Mark S. Lowenthal, Cory Bystrom
Harmonization of diagnostic test results is fundamental to the effective use of laboratory testing in the diagnosis, treatment and monitoring of disease. Formal

Software

NIST Mass and Fragment Calculator Software

The NIST Mass and Fragment Calculator is a program written in Visual Basic which calculates the mass of an input peptide or protein sequence along with m/z ions

Isotope Enrichment Calculator

The NIST Isotope Enrichment Calculator is a program written in Visual Basic which determines the percentage of 15N enrichment of stable isotope-labeled peptides

Contacts

Group Leader