Tynan, K.
, Mouchahoir, C.
, Lowenthal, M.
and Phinney, K.
(2026),
Purification of Double-­Stranded RNA Impurities From In Vitro-­Transcribed mRNA Using Size-­Exclusion Chromatography, Biomedical Chromatography, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=961659 (Accessed June 25, 2026)
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Purification of Double-Stranded RNA Impurities From In Vitro-Transcribed mRNA Using Size-Exclusion Chromatography
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Author(s)
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Abstract
dsRNA is a highly immunogenic byproduct generated during in vitro transcription of mRNA, leading to unwanted immunos- timulatory responses and reduced vaccine efficacy. Removal of dsRNA impurities from mRNA is challenging, as they share sim- ilar physicochemical properties. Here, we present a lab-scale size-exclusion chromatography method that effectively separates dsRNA impurities from mRNA. This method achieves baseline resolution between dsRNA impurities and mRNA (resolution factor of 1.6) while maintaining high recovery of the target mRNA (81.8%). This work introduces a novel chromatographic strat- egy for the removal of dsRNA from mRNA therapeutics.Citation
Biomedical Chromatography
Volume
40
Issue
7
Pub Type
Journals
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Keywords
dsRNA, hydrodynamic chromatography, in vitro transcription, mRNA, purification, size-exclusion chromatography
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Created June 17, 2026, Updated June 24, 2026