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Displaying 1 - 18 of 18

Extracellular and Intracellular Chorismate Mutases From Mycobacterium tuberculosis H 37 R v : Characterization of the Hypothetical Gene Products Rv1885c and Rv0948c

October 12, 2021
Author(s)
Sung Kim, Sathyavelu K. Reddy, G B. Vasquez, B C. Nelson, A M. Davis, S. Patterson, Prasad T. Reddy
Two ORFs Rv1885c and Rv0948c from the genome of Mycobacterium tuberculsosis H37Rv were identified as putative chorismate mutases. We reported earlier that Rv1885c is indeed a chorismate mutase (CM) with a 33 amino acid cleavable signal sequence in a

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Prasad T. Reddy, Robert G. Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Robert Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen, Zvi Kelman, Prasad T. Reddy
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost

Extreme expression of DNA repair protein apurinic/apyrimi-dinic endonuclease I (APE1) in human breast cancer as measured by liquid-chromatography isotope-dilution tandem mass spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Prasad T. Reddy, Miral M. Dizdar
Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA re-pair protein and plays other important roles. Increased lev-els of APE1 in cancer have been reported. However, avail-able methods to measure APE1 levels are indirect and not quantitative. We

Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements

July 26, 2015
Author(s)
Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday, Miral M. Dizdar
Oxidatively induced DNA damage is caused in living organisms by a variety of damaging agents, resulting in the formation of a multiplicity of lesions, which are mutagenic and cytotoxic. Unless repaired by DNA repair mechanisms before DNA replication, DNA

Methods to Characterize Ricin for the Development of Reference Materials

July 1, 2006
Author(s)
Sung Kim, Diane K. Hancock, Lili Wang, Kenneth D. Cole, Prasad T. Reddy
Ricin is an abundant protein from the castor bean plant Ricinus communis. Because of its high toxicity and the simplicity of producing mass quantities, ricin is considered a biological terrorism agent. We have characterized ricin extensively with a view to

The HI0073/HI0074 Protein Pair From Haemophilus Influenzae is a Member of a New Nucleotidyltransferase Family: Structure, Sequence Analyses, and Solution Studies

February 1, 2003
Author(s)
C Lehmann, Kap Lim, V R. Chalamasetty, W Krajewski, E Melamud, Andrey Galkin, A Howard, Z Kelman, Prasad T. Reddy, A Murzin, O Herzberg
The crystal structure of HI0074 from Haemophilus influenzae, a protein of unknown function, has been determined at a resolution of 2.4 . The molecules form an up-down four-helix bundle, and associate into homodimers. The fold is most closely related to the

Structure of HI1333 (YhbY), a Putative RNA-Binding Protein From Haemophilus Influenzae

November 1, 2002
Author(s)
M A. Willis, W Krajewski, V R. Chalamasetty, Prasad T. Reddy, A J. Howard, O Herzberg
The structures of a number of small [alpha]/[Beta] RNA-binding proteins with diverse biological functions are known1. Their topologies and the locations of the RNA-binding sites vary considerably, consistent with the plasticity of RNA due to base-pair

Prokaryotic Expression, Purification, and Reconstitution of Biological Activities (Antiprotease, Antitumor and Heparin-Binding) for Tissue Factor Pathway Inhibitor- 2

October 5, 2000
Author(s)
C N. Rao, Prasad T. Reddy, D J. Reeder, Yonglin Liu, S M. Stack, W Kisiel, D T. Woodley
We report the expression of tissue factor pathway inhibitor-2 (TFPI-2) (also known as PP-5, placental protein-5; MSPI, matrix-associated serine protease inhibitor) in E. coli as a 25 kDa non-glycosylated protein with a glycine substituted for aspartic acid