The 1.30 Angstrom Resolution Structure of Bascillus Subtillis Chorismate Mutase Catalytic Homotrimer

Published

June 1, 2000

Author(s)

Jane E. Ladner, Prasad T. Reddy, A M. Davis, M Tordova, A Howard, G L. Gilliland

Abstract

The crystal structure of the Bacillus subtilis chorismate mutase, an enzyme of the aromatic amino acids biosynthetic pathway, was determined to 1.30- resolution. The structure of the homotrimer was determined by molecular replacement using orthohombic crystals of space group P2₁2₁2₁ with unit-cell parameters a =52.2, b = 83.8, c = 86.0 . The ABC trimer of the monoclinic crystal structure [Chook et al. (1994) J. Mol. Biol. 240, 476-500] was used as the starting model. The final coordinates are composed of three complete polypeptide chains of 127 amino-acid residues. In addition, there are nine sulfate ions, five glycerol molecules and 424 water molecules clearly visible in the structure. This structure was refined with aniosotropic temperature factors, has excellent geometry, and a crystallographic R factor of 0.169 with an R_free of 0.236. The three active sites of the macromolecule are at the subunit interfaces, with residues from two subunits contributing to each site. This orthorhombic crystal form was grown using ammonium sulfate as the precipitant; glycerol was used as a cryoprotectant during data collection. A glycerol molecule and sulfate ion in each of the active sites was found mimicking a transition state analog. In this structure, the C-terminal tails of the subunits of the trimer are hydrogen bonded to residues of the active site of neighboring trimers in the crystal and thus cross-link the molecules in the crystal lattice.

Citation

ACTA Crystallographica Section D-Biological Crystallography

Volume

56

Issue

Part 6

Pub Type

Journals

Keywords

aromatic amino acid synthesis, chorismate mutase, protein crystal structure, transition state mimick, x-ray crystallography

Citation

Ladner, J. , Reddy, P. , Davis, A. , Tordova, M. , Howard, A. and Gilliland, G. (2000), The 1.30 Angstrom Resolution Structure of Bascillus Subtillis Chorismate Mutase Catalytic Homotrimer, ACTA Crystallographica Section D-Biological Crystallography (Accessed April 23, 2024)