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Search Publications by: Jason Kralj (Fed)

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Displaying 1 - 14 of 14

Report from the 2022 NIST Rapid Microbial Testing Methods (RMTM) Workshop

August 15, 2023
Author(s)
Stephanie Servetas, Nancy Lin, Nadratun Chowdhury, Scott Jackson, Jason Kralj, Sheng Lin-Gibson, Melody Sanders, Tara Eskandari
Safety and quality of advanced therapies, including cellular, gene, and tissue-engineered medical products, is paramount for success of these products. Sterility assurance testing confirming the absence of microbial contamination in advanced therapy

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Author(s)
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Performance of NIST SRM(R) 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

February 10, 2022
Author(s)
Jessica Willis, Mano Sivaganesan, Richard Haugland, Jason Kralj, Stephanie Servetas, Monique Hunter, Scott Jackson, Orin Shanks
Fecal pollution remains a significant challenge for recreational water quality management worldwide. In response, there is a growing interest in the use of real-time quantitative PCR (qPCR) methods to achieve same-day notification of recreational water

Capturing Rare Cells From Blood Using a Packed Bed of Custom-Synthesized Chitosan Microparticles

July 1, 2013
Author(s)
Chandamany Arya, Jason Kralj, Kunqiang Jiang, Matt S. Munson, Thomas Forbes, Don L. DeVoe, Srinivasa R. Raghavan, Samuel Forry
Uniform multifunctional chitosan microparticles were produced in large numbers using a simple and inexpensive microtubing arrangement. The particles were functionalized through both physical encapsulation of carbon black, to attenuate autofluorescence, and

Simple device for rare cell capture from whole blood

September 26, 2012
Author(s)
Jason G. Kralj, Samuel P. Forry, Matt S. Munson, Thomas P. Forbes, Chandamany Arya, Lynn Sorbara, Alessandro Tona, Sudhir Srivastava
We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics that can provide biologists with the capabilities of the monolithic devices. We characterized the capture of MCF-7 cells

Image-Based Feedback Control for Real-Time Sorting of Microspheres in a Microfluidic Device

January 1, 2010
Author(s)
Matt S. Munson, James Spotts, Antti Niemisto, Jyrki Selinummi, Jason G. Kralj, Marc L. Salit, Adrian Ozinsky
We have developed a fully automated control system to distribute user-defined numbers of antibody-functionalized fluorescent beads (scalable from tens to hundreds) to addressable assay chambers within a microfluidic PDMS device. We report an automated bead

T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements

April 7, 2009
Author(s)
Jason G. Kralj, Audrey Player, Hope Sedrick, Matt S. Munson, David Petersen, Samuel P. Forry, Paul Meltzer, Ernest Kawasaki, Laurie E. Locascio
We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microarray

Multiplexed Detectorless Electrophoresis

December 15, 2008
Author(s)
David J. Ross, Jason G. Kralj
A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common