NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: Jason Kralj (Fed)

Search Title, Abstract, Conference, Citation, Keyword or Author
Displaying 1 - 17 of 17

NIST 2025 Rapid Microbial Testing Methods Workshop Report

September 26, 2025
Author(s)
Jason Kralj, Kirsten Parratt, Tyler Laird, Stephanie Servetas, Nancy Lin, Dawn Henke, Scott Jackson
In 2020, the National Institute of Standards and Technology (NIST)-led Rapid Microbial Testing Methods (RMTM) Consortium was established to develop standards and measurement-based solutions to facilitate the adoption of RMTMs in advanced therapy products

Report from the 2023 NIST-Hosted Workshop on Collaborative Efforts to Enable Adoption of Rapid Microbial Testing Methods for Advanced Therapy Products

September 26, 2025
Author(s)
Stephanie Servetas, Dawn Henke, Jason Kralj, Kirsten Parratt, Scott Jackson, Nancy Lin
On April 25, 2023, the National Institute of Standards and Technology (NIST) hosted a one-day hybrid workshop focused on bringing together organizations and working groups with the common goal of enabling validation and implementation of rapid microbial

NIST 2024 Rapid Microbial Testing Methods Workshop Report

September 25, 2025
Author(s)
Jason Kralj, Nancy Lin, Kirsten Parratt, Stephanie Servetas, Dawn Henke, Scott Jackson
The NIST-led Rapid Microbial Testing Methods (RMTM) Consortium launched in 2020 to develop standards and measurement-based solutions to advance the use of RMTMs in advanced therapy products. NIST hosted 4th annual workshop on RMTMs to update the community

Report from the 2022 NIST Rapid Microbial Testing Methods (RMTM) Workshop

August 15, 2023
Author(s)
Stephanie Servetas, Nancy Lin, Nadratun Chowdhury, Scott Jackson, Jason Kralj, Sheng Lin-Gibson, Melody Sanders, Tara Eskandari
Safety and quality of advanced therapies, including cellular, gene, and tissue-engineered medical products, is paramount for success of these products. Sterility assurance testing confirming the absence of microbial contamination in advanced therapy

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Author(s)
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Performance of NIST SRM(R) 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

February 10, 2022
Author(s)
Jessica Willis, Mano Sivaganesan, Richard Haugland, Jason Kralj, Stephanie Servetas, Monique Hunter, Scott Jackson, Orin Shanks
Fecal pollution remains a significant challenge for recreational water quality management worldwide. In response, there is a growing interest in the use of real-time quantitative PCR (qPCR) methods to achieve same-day notification of recreational water

Capturing Rare Cells From Blood Using a Packed Bed of Custom-Synthesized Chitosan Microparticles

July 1, 2013
Author(s)
Chandamany Arya, Jason Kralj, Kunqiang Jiang, Matt S. Munson, Thomas Forbes, Don L. DeVoe, Srinivasa R. Raghavan, Samuel Forry
Uniform multifunctional chitosan microparticles were produced in large numbers using a simple and inexpensive microtubing arrangement. The particles were functionalized through both physical encapsulation of carbon black, to attenuate autofluorescence, and

Simple device for rare cell capture from whole blood

September 26, 2012
Author(s)
Jason G. Kralj, Samuel P. Forry, Matt S. Munson, Thomas P. Forbes, Chandamany Arya, Lynn Sorbara, Alessandro Tona, Sudhir Srivastava
We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics that can provide biologists with the capabilities of the monolithic devices. We characterized the capture of MCF-7 cells

Image-Based Feedback Control for Real-Time Sorting of Microspheres in a Microfluidic Device

January 1, 2010
Author(s)
Matt S. Munson, James Spotts, Antti Niemisto, Jyrki Selinummi, Jason G. Kralj, Marc L. Salit, Adrian Ozinsky
We have developed a fully automated control system to distribute user-defined numbers of antibody-functionalized fluorescent beads (scalable from tens to hundreds) to addressable assay chambers within a microfluidic PDMS device. We report an automated bead

T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements

April 7, 2009
Author(s)
Jason G. Kralj, Audrey Player, Hope Sedrick, Matt S. Munson, David Petersen, Samuel P. Forry, Paul Meltzer, Ernest Kawasaki, Laurie E. Locascio
We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microarray

Multiplexed "Detectorless" Electrophoresis

December 14, 2008
Author(s)
David Ross, Jason Kralj
A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common
Was this page helpful?