Certification of Standard Reference Material® 2917: Plasmid DNA for Fecal Indicator Detection and Identification

Published

December 6, 2021

Author(s)

Jason Kralj, Stephanie Servetas, Monique Hunter, Blaza Toman, Scott Jackson

Abstract

Standard Reference Material (SRM) 2917 is intended for calibrating qPCR-based assays of fecal indicator bacteria (FIB) for assessing water quality. A unit of SRM 2917 consists of 6 Levels of linearized plasmid DNA. The length of DNA is approximately 4.4 kb; it contains 13 single-copy PCR target regions. The 6 Levels span a log10 dilution series from approximately (5 to 500,000) plasmid copy per uL. Each Level is suspended in 1× TE buffer at pH 8 with approximately 10 ng/uL yeast tRNA. Approximately 0.2 mL of each Level were bottled in 1.5 mL skirted centrifuge vials. In total, approximately 1000 units were generated. The material is both stable and should be stored at 4 °C. The production, analytical methods, statistical evaluation, and certified values of this SRM are described herein.

Citation

Special Publication (NIST SP) - 260-221

Report Number

260-221

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.260-221

Local Download

Keywords

Water quality, fecal indicator, qPCR, Plasmid DNA, ddPCR

Citation

Kralj, J. , Servetas, S. , Hunter, M. , Toman, B. and Jackson, S. (2021), Certification of Standard Reference Material® 2917: Plasmid DNA for Fecal Indicator Detection and Identification, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-221, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933616 (Accessed April 25, 2024)