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Search Publications by: Darwin R. Reyes (Fed)

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Displaying 1 - 25 of 57

Recommendations on fit-for-purpose criteria to establish a quality management for microphysiological systems (MPS) and for monitoring of their reproducibility

May 14, 2024
Author(s)
David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, MONICA PIERGIOVANNI, Benjamin Freedman, Adrian Keong, Darwin Reyes-Hernandez, Hendrik Erfurth, Peter Loskill, Pelin Candarlioglu, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn Stacey, Marcel Leist
Cell culture technology has seen great innovations and progress in teh 21st centuryClassical single-cell and monolayer models have been replaced by more complex 3D models (e.g., reaggregates, spheroids, organoids) to improve the predictivity of in vitro

From animal testing to in vitro systems: advancing standardization in microphysiological systems

February 15, 2024
Author(s)
Darwin Reyes-Hernandez, Mandy Esch, Lorna Ewart, Rohollah Nasiri, Anna Herland, Carolina Lucchesi, Kyung Sung, MONICA PIERGIOVANNI, Jelena Vukasinovic, James Shoemaker, Nureddin Ashammakhi
Limitations with cell cultures and experimental animal-based studies have had the scientific and industrial communities searching for new approaches that can provide reliable human models for applications such as drug development, toxicological assessment

Heart-on-a-Chip Systems: Disease Modeling and Drug Screening Applications

February 14, 2024
Author(s)
Derrick Butler, Darwin Reyes-Hernandez
Cardiovascular disease (CVD) is the leading cause of death worldwide, casting a substantial economic footprint and burdening the global healthcare system. Historically, pre-clinical CVD modeling and therapeutic screening has been performed using animal

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

November 18, 2023
Author(s)
Karina Torres Castro, Katherine Acuna-Umana, Leonardo Lesser-Rojas, Darwin Reyes-Hernandez
Blood is a complex sample comprised mostly of plasma, red blood cells (RBCs), and other cells whose concentrations correlate to physiological or pathological health conditions. There are also many blood-circulating biomarkers, such as circulating tumor

Overcoming Technological Barriers in Microfluidics: Leakage Testing

September 1, 2022
Author(s)
Vania Silverio, Suvajyoti Guha, Armelle Keiser, Rucha Natu, Darwin Reyes-Hernandez, Henne van Heeren, Nicolas Verplanck, Luke Herbertson
The miniaturization of laboratory procedures for Lab-on-Chip (LoC) devices and translation to various platforms such as single cell analysis or Organ-on-Chip (OoC) systems are revolutionizing the life sciences and biomedical fields. As a result

Protocols for leakage testing

June 1, 2022
Author(s)
Henne van Heeren, Matthew Davies, Armelle Keiser, Rudy Lagrauw, Darwin Reyes-Hernandez, Vania Silverio, Nicolas Verplanck
This document is written to serve as a guideline for the testing of microfluidic devices, which are devices designed to manipulate fluids that are confined in a small volume. For instance, a channel with at least one dimension smaller than 1 mm is a

Amontons-Coulomb-like slip dynamics in acousto-microfluidics

March 22, 2022
Author(s)
Aurore F. Quelennec, Jason J. Gorman, Darwin Reyes-Hernandez
Electroacoustic technologies, which employ acoustic waves to manipulate and detect bioparticles, have been increasingly used, with a great deal of success, in life sciences. Particle manipulation or measurement capabilities of surface acoustic wave-based

Accelerating Innovation and Commercialization Through Standardization of Microfluidic-Based Medical Devices

January 5, 2021
Author(s)
Darwin R. Reyes-Hernandez, Henne van Heeren, Suvajyoti Guha, Luke Herbertson, Alexios P. Tzannis, Jens Ducr?e, Hugo Bissig, Holger Becker
The microfluidics industry has grown steadily over the last 5 years worldwide, with the market for microfluidic medical devices experiencing a compound growth rate of 18%. The number of submissions to regulatory agencies such as the U.S. Food & Drug

Frequency tunable label-free surface acoustic wave-based flow sensor

October 7, 2020
Author(s)
Aurore F. Quelennec, Jason J. Gorman, Darwin Reyes-Hernandez
We present a label-free SAW-based flow sensor with enhanced precision and repeatability. This sensor improves the signal-to-noise ratio by one order of magnitude (dB scale) at an optimized frequency, and lowers the measurable flow rate to 5 μL/min (from

Label-free surface acoustic wave-based embedded flow sensor

October 27, 2019
Author(s)
Aurore F. Quelennec, Jason J. Gorman, Darwin Reyes-Hernandez
This paper presents a calibration/label-free flow sensor embedded in a microfluidic system. This sensor is based on surface acoustic waves, where the acoustic intensity is dependent on the flow rate of the propagating medium. The range of flow rates

Live quantification of cell viability via neutral red uptake using lens-free imaging

October 27, 2019
Author(s)
Brian J. Nablo, Jungjoon Ahn, Kiran Bhadriraju, Jong M. Lee, Darwin Reyes-Hernandez
We present the quantification of cell viability during neutral red (NR) uptake with a compact lens-free system utilizing two light sources. Conventionally, the NR uptake assay determines cell viability based on the accumulation of NR within lysosomes and

Proceedings of the First Workshop on Standards for Microfluidics

January 28, 2019
Author(s)
Darwin R. Reyes-Hernandez, Henne van Heeren
In the last two decades, the microfluidics/lab-on-a-chip field has evolved from the concept of micro total analysis systems, where systems with integrated pretreatment and analysis of chemicals were envisioned, to what is known today as lab-on-a-chip

The 2016 edition of the Art in Science of MicroTAS

April 21, 2017
Author(s)
Darwin R. Reyes-Hernandez
Dublin, Ireland beheld The 20th International Conference of Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) from October 23 to 27, 2016. The conference not only included a great technical program, but also outreach activities, an

FINE-TUNING ELECTRICAL FLOW RATE SENSING IN DEFORMABLE CHANNELS

October 9, 2016
Author(s)
Pengfei Niu, Brian J. Nablo, Kiran Bhadriraju, Darwin Reyes-Hernandez
Here we present conclusive proof that when using electrical impedance to measure volumetric flow rate in polydimethylsiloxane (PDMS) microchannels a considerable fraction of the change in impedance, due to change in flow rate, is caused by the variation in