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Search Publications by: Pawel Jaruga (Fed)

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Displaying 76 - 100 of 111

Cellular DNA Biomarkers for the Safety of Tissue-Engineered Medical Products Using Artificial Skin as a Model

October 16, 2008
Author(s)
H Rodriguez, C D. O'Connell, Peter E. Barker, M. Dizdaroglu, Donald H. Atha, Pawel Jaruga, M Birincioglu, M A. Marino, P McAndrew
Fundamental to the safety of many tissue-engineered medical products is how cells respond to a given polymer when implanted into the body. Assurance of cellular stability during the manufacturing, storage and shipment of such products is crucial to achieve

Structural alterations in breast stromal and epithelial DNA: the influence of 8,5'-cyclo-2'-deoxyadenosine

June 1, 2006
Author(s)
K. M. Anderson, Pawel Jaruga, C. R. Ramsey, N. K. Gilman, V. M. Green, S. W. Rostad, J. T. Emerman, M Miral Dizdar, D. C. Malins
(5'S)-8,5'-Cyclo-2'-deoxyadenosine (S-cdA), which arises from the reaction of the hydroxyl radical (*OH) with 2'-deoxyadenosine in DNA, is a lesion comprising a base-sugar linkage that distorts the DNA backbone. This structure impedes transcription and

Repair of Formamidopyrimidines in DNA Involves Different Glycosylases - Role of the OGG1, NTH1, and NEIL1 Enzymes

December 9, 2005
Author(s)
J Hu, N de Souza-Pinto, K Haraguchi, Barbara A. Hogue, Pawel Jaruga, M M. Greenberg, Miral M. Dizdar, V. Bohr
2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA), are formed abundantly in DNA of cultured cells or tissues exposed to ionizing radiation or to other free radical-generating systems. We show here that FapyG

Molecular Biomarkers Used to Detect Cellular/Genetic Damage in Tissue-Engineered Skin

January 1, 2004
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, H Rodriguez
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, Tissue engineered skin (TestSkin II) was obtained, separated into its two cellular layers

Genomic DNA of Nostoc Commune (Cyanobacteria) Becomes Covalently Modified During Long-Term (Decades) Desiccation But Is Protected From Oxidative Damage and Degradation

June 15, 2003
Author(s)
B Shirkey, N J. McMaster, Sue C. Smith, D J. Wright, H Rodriguez, Pawel Jaruga, M Birincioglu, R F. Helm, M Potts
Genomic DNA of Nostoc commume (Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N-phenacylthiazolium bromide; a reagent that cleaves DNA- and protein

Arabidopsis Thaliana Ogg1 Protein Excises 8-Hydroxyguanine and 2,6- Diamino-4-hydroxy-5-formamidopyrimidine from Oxidative Damaged DNA Containing Multiple Lesions

March 18, 2003
Author(s)
T Morales-Ruiz, M Birincioglu, Pawel Jaruga, H Rodriguez, T. Roldan-Arjona, Miral M. Dizdar
A functional homologue of eukaryotic Ogg1 proteins in the model plant Arabidopsis thaliana has recently been cloned, isolated, and characterized [Garcia-Ortiz, M.V., Ariza, R.R., and Roldan-Arjona, T. (2001) Plant Mol. Biol. 47, 795-804]. This enzyme

Biomarkers Used to Detect Genetic Damage in Tissue-Engineered Skin

January 1, 2003
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, M. Dizdaroglu, H Rodriguez
The goal of the research was to help identify cellular biomarkers and assess measurement technologies that will help manufacturers determine how cells react to inflammatory stimuli from different polymer matrixes and assure that tissue-engineered medical

Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin

January 1, 2003
Author(s)
H Rodriguez, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell, M. Dizdaroglu
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, our laboratory is responding to the need for test methods used to assess the safety and

Biomarkers of TP53 Mutation and Y-Chromosome Loss Used to Detect Cellular/Genetic Damage in Tissue Engineered Skin

September 1, 2002
Author(s)
H Rodriguez, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell
Tissue engineering is an emerging area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. Fundamentals to many tissue-engineered

Free Radical-Induced Damage to DNA: Mechanisms and Measurement

June 1, 2002
Author(s)
M. Dizdaroglu, Pawel Jaruga, M Birincioglu, H Rodriguez
Free radicals are produced in cells by cellular metabolism and by exogenous agents. These species react with biomolecules in cells including DNA. The resulting damage to DNA which is also called oxidative damage to DNA, is implicated in mutagenesis

Identification and Characterization of a Human DNA Glycosylase for Repair of Modified Bases in Oxidatively Damaged DNA

March 19, 2002
Author(s)
T. K. Hazra, T. Izumi, I Boldough, B Imhoff, Y W. Kow, Pawel Jaruga, Miral M. Dizdar, Somenath Mitra
8-oxoguanine (8-oxoG), ring-opened purines (formamidopyrimidines or Fapys), and other oxidized DNA base lesions generated by reactive oxygen species, are often mutagenic and toxic, and have been implicated in the etiology of many diseases including cancer