Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin

Published

January 1, 2003

Author(s)

H Rodriguez, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell, M. Dizdaroglu

Abstract

To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, our laboratory is responding to the need for test methods used to assess the safety and performance of tissue-engineered materials. Specifically, we are identifying cellular biomarkers that could be used during the in vitro development phase of tissue-engineered materials to ensure that cells have not undergone any inflammatory response during the development or shipment of the product. Using GC/MS technology, we have screened for a total of five genomic modified base DNA biomarkers in tissue-engineered skin and compared the levels to control cells, neonatal fibroblasts and neonatal keratinocytes. No significant level of damage was detected compared to control cells. Biomarker programs such as this can provide the basis for an international reference standard of cellular biomarkers that can aid in the development and safety of tissue engineered medical products.

Proceedings Title

Tissue Engineering, Stem Cells, and Gene Therapies

Volume

534

Conference Dates

September 19-22, 2002

Conference Location

Antalya,

Conference Title

9th International Symposium on Biomedical Science and Technology

Pub Type

Conferences

Keywords

DNA damage, oxidative, tissue engineering

Citation

Rodriguez, H. , Jaruga, P. , Birincioglu, M. , Barker, P. , O'Connell, C. and Dizdaroglu, M. (2003), Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin, Tissue Engineering, Stem Cells, and Gene Therapies, Antalya, (Accessed December 14, 2024)