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Search Publications by: Pawel Jaruga (Fed)

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Displaying 51 - 75 of 111

Inhibition of DNA glycosylases via small molecule purine analogs

December 9, 2013
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Pawel Jaruga, Amanda K. McCullough, R. S. Lloyd
Following the formation of oxidatively-induced DNA damage, several DNA glycosylases are required to initiate repair of the base lesions that are formed. Recently, NEIL1 and other DNA glycosylases, including OGG1 and NTH1 were identified as potential

Identification and Quantification of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Cells by Liquid Chromatography/Isotope-Dilution Tandem Mass Spectrometry

July 29, 2013
Author(s)
Guldal Kirkali, Pawel Jaruga, Prasad T. Reddy, Alessandro Tona, Li Mengxia, David M. Wison III, M Miral Dizdar, Bryant C. Nelson
Unless repaired, DNA damage can drive mutagenesis or cell death. DNA repair proteins may therefore be used as biomarkers in disease detection or therapeutic response estimation. Thus, the accurate measurement of DNA repair protein expression is of

Tools and Approaches for the Assessment of Nanomaterial Induced Oxidative DNA Damage

May 13, 2013
Author(s)
Elijah J. Petersen, Bryce Marquis, Pawel Jaruga, M Miral Dizdar, Bryant C. Nelson
Hyphenated mass spectrometry techniques have been employed as one of the primary analytical tools for investigating the effects of ionizing radiation, chemical/biological carcinogens, and oxygen derived free radicals on the induction and subsequent repair

Identification and Quantification of Human DNA Repair Protein NEIL1 by Liquid Chromatography/Isotope-Dilution Tandem Mass Spectrometry

December 26, 2012
Author(s)
M Miral Dizdar, Pawel Jaruga, Guldal Kirkali, Bryant C. Nelson, Gamze Tuna, Prasad T. Reddy
Accumulated evidence points to DNA repair capacity as an important factor in cancer and other diseases. DNA repair proteins are promising drug targets and are emerging as prognostic and therapeutic biomarkers. Thus, the knowledge of the overexpression or

Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine.

July 11, 2012
Author(s)
M Miral Dizdar, Pawel Jaruga, Susan Duclos, Pierre Aller, Susan Wallace, Susan Doublie
Formamidopyrimidine-DNA glycosylase (Fpg; MutM) is a DNA repair enzyme widely distributed in bacteria. Fpg recognizes and excises oxidatively modified purines, 4,6-diamino-5-formamidopyrimidine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxoguanine

Tools and Approaches for the Assessment of Nanomaterial Induced Oxidative DNA Damage

June 20, 2012
Author(s)
Elijah J. Petersen, Bryce J. Marquis, Pawel Jaruga, M Miral Dizdar, Bryant C. Nelson
Hyphenated mass spectrometry techniques have been employed as one of the primary analytical tools for investigating the effects of ionizing radiation, chemical/biological carcinogens, and oxygen derived free radicals on the induction and subsequent repair

Identification and quantification of DNA repair proteins by liquid chromatography/isotope-dilution tandem mass spectrometry using 15N-labeled whole proteins as internal standards

May 30, 2012
Author(s)
M Miral Dizdar, Prasad T. Reddy, Guldal Kirkali, Gamze Tuna, Bryant C. Nelson, Pawel Jaruga
Oxidatively induced DNA damage is implicated in disease, unless it is repaired by DNA repair. DNA repair proteins may be used as early detection and therapeutic biomarkers in cancer and other diseases. For this purpose, the measurement of the expression

Mechanisms of free radical-induced damage to DNA

April 1, 2012
Author(s)
M Miral Dizdar, Pawel Jaruga
Endogenous and exogenous sources cause free radical-induced DNA damage in living organisms by a variety of mechanisms. The highly reactive hydroxyl radical reacts with the heterocyclic DNA bases and the sugar moiety near or at diffusion-controlled rates by

Oxidatively Induced DNA Damage and Cancer

December 30, 2011
Author(s)
M Miral Dizdar, Pawel Jaruga
Oxidatively induced DNA damage is caused by endogenous and exogenous sources in living organisms. Many resulting DNA lesions are mutagenic and lead to mutations commonly found in cancer. Repairs mechanisms exist to repair this type of DNA damage

Copper oxide nanoparticle mediated DNA damage in terrestrial plant models

December 22, 2011
Author(s)
Bryant C. Nelson, Donald H. Atha, Elijah J. Petersen, Huanhua Wang, Danielle Cleveland, Richard D. Holbrook, Pawel Jaruga, M Miral Dizdar, Baoshan Xing
Engineered nanoparticles, due to their unique electrical, mechanical and catalytic properties, are presently found in many commercial products and will be intentionally or inadvertently released at increasing concentrations into the natural environment

Evidence for upregulated repair of oxidatively induced DNA damage in human colorectal cancer

September 15, 2011
Author(s)
M Miral Dizdar, Pawel Jaruga, Prasad T. Reddy, Guldal Kirkali, Didem Keles, Gulgun Oktay, Aras E. Canda
Molecular pathways that play a role in the development of colorectal cancer involve multiple genetic changes in cancer-related genes that may be caused by overproduction of oxygen-derived species including free radicals, which are capable of damaging DNA

Stable isotope-labeling of DNA repair proteins, and their purification and characterization

July 1, 2011
Author(s)
M Miral Dizdar, Pawel Jaruga, Prasad T. Reddy, Bryant C. Nelson, Mark S. Lowenthal
Reduced DNA repair capacity is associated with increased risk for a variety of disease processes including carcinogenesis. Thus, DNA repair proteins have the potential to be used as important predictive, prognostic and therapeutic biomarkers in cancer and

A Major Role for Non-Enzymatic Antioxidant Processes in the Radioresistance of Halobacterium salinarum

April 1, 2011
Author(s)
M Miral Dizdar, Pawel Jaruga, Courtney K. Robinson, Kim Webb, Amardeep Kaur, Nitin S. Baliga, Allen Place, Jocelyne DiRuggiero
Oxidative stress occurs when the generation of reactive oxygen species (ROS) exceeds the capacity of the cell’s endogenous systems to neutralize them. We analyzed the oxidative stress responses and cellular damage of the archaeon Halobacterium salinarum

The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo

March 16, 2010
Author(s)
Minin Liu, Viswanath Bandaru, Jeffrey Bond, Pawel Jaruga, Xiaobei Zhao, Plamen P. Christov, Cynthia Burrows, Carmelo J. Rizzo, Miral M. Dizdar, Susan Wallace
To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases and to initiate the Base Excision Repair (BER) pathway. Recently, three DNA glycosylases were identified in mammals that are

The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate specificities from their Escherichia coli counterparts

February 4, 2010
Author(s)
Yin Guo, Viswanath Bandaru, Pawel Jaruga, Xiaobei Zhao, Cynthia Burrows, Shigenori Iwai, Miral M. Dizdar, Jeffrey Bond, Susan Wallace
The DNA glycosylases function in the first step of the base excision repair process that is responsible for removing endogenous oxidative purine and pyrimidine damages from DNA. The DNA glycosylases that remove oxidized DNA bases fall into two general

Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase

September 1, 2009
Author(s)
Viktoriya Sidorenko, Arthur P. Grollman, Pawel Jaruga, Miral M. Dizdar, Dmitry Zharhov
Human 8-oxoguanine-DNA-glycosylase (OGG1) efficiently removes mutagenic 8-oxoguanine (8-oxoGua) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) when paired with cytosine in damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to GT

Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases

May 1, 2009
Author(s)
Scott D. Kathe, Ramiro Barrantes-Reynolds, Pawel Jaruga, Michael Newton, Cynthia Burrows, Viswanath Bandaru, Miral M. Dizdar, Jeffrey Bond, Susan Wallace
Formamidopyrimidine DNA glycosylase (Fpg) and endonuclease VIII (Nei) share an overall common three dimensional structure and primary amino acid sequence in conserved structural motifs but have different substrate specificities, with bacterial Fpg proteins

Measurement of formamidopyrimidines in DNA

December 15, 2008
Author(s)
Pawel Jaruga, Guldal Kirkali, Miral M. Dizdar
Formamidopyrimidines, 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), are among major lesions in DNA generated by hydroxyl radical attack, UV radiation or photosensitization in vitro and in vivo