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Search Publications by: Karen W. Phinney (Fed)

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Displaying 26 - 50 of 94

Mass Spectral Library Quality Assurance by Inter-Library Comparison

April 2, 2017
Author(s)
William E. Wallace, Weihua Ji, Dmitrii V. Tchekhovskoi, Karen W. Phinney, Stephen E. Stein
A method to discover and correct errors in mass spectral libraries is described. Comparing across a set of highly curated reference libraries compounds that have the same chemical structure quickly identifies entries that are outliers. In cases where three

A new approach to quantification of mAb aggregates using peptide affinity probes

February 10, 2017
Author(s)
Crystal Cheung, Kyle Anderson, Pooja Patel, Keale Cade, Karen W. Phinney, Illarion Turko
Using mAbs as therapeutic molecules is complicated by the propensity of mAbs to aggregate at elevated concentrations, which can lead to a variety of adverse events in treatment. Here, we describe a proof-of-concept for new methodology to detect and

Identification of novel N-glycosylation sites at non-canonical protein consensus motifs

June 14, 2016
Author(s)
Mark S. Lowenthal, Kiersta S. Davis, Lisa E. Kilpatrick, Catherine A. Mouchahoir, Karen W. Phinney
N-glycosylation is well known to occur at asparagine residues in the canonical consensus sequence N-X-S/T, but has also been identified at a small number of N-X-C motifs including the Asn491 residue of human serotransferrin. Here we report additional novel

QUANTITY: An Isobaric Tag for Quantitative Glycomics

November 30, 2015
Author(s)
Shuang Yang, Meiyao Wang, Lijun Chen, Illarion Turko, Karen W. Phinney, Shuwei Li
We describe the design and synthesis of a novel set of iso-baric tags for quantitative glycan profiling, which will have broad applications in carbohydrate based biomarker dis-covery, therapeutic protein characterization, and vaccine development.

Quantification of Borrelia burgdorferi membrane proteins in human serum: a new concept for detection of bacterial infection

November 17, 2015
Author(s)
Sao F. Cheung, Kyle Anderson, Kenia Benitez, Mark J. Soloski, John N. Aucott, Karen W. Phinney, Illarion Turko
The low abundance of bacterial proteins in human serum upon infection imposes a challenge for the early proteomic detection of bacterial infection. To address this challenge, we propose to take advantage of detecting membrane proteins released from

QconCAT: Internal Standard for Protein Quantification

October 21, 2015
Author(s)
Kerry M. Bauer, Karen W. Phinney, Illarion V. Turko
Protein quantification based on stable-isotope labeling mass spectrometry (SIL-MS) involves adding known quantities of stable-isotope labeled internal standards into biological samples. The internal standards are analogous to analyte molecules and

Determination of the Primary Sequence/ Structure

October 15, 2015
Author(s)
Catherine A. Mouchahoir, Mellisa Ly, Michaella Levy, Lisa E. Kilpatrick, Scott C. Lute, Karen W. Phinney, Lisa Marzilli, Kurt A. Brorson, Michael T. Boyne, Darryl Davis, John E. Schiel
The primary sequence of a protein, including therapeutic monoclonal antibodies (mAbs), is a critical quality attribute that determines a great deal of its functionality and stability. Significant effort is devoted to determining the complete amino acid

Standardizing 25-Hydroxyvitamin D values from the Canadian Health Measures Survey

September 30, 2015
Author(s)
Kurtis Sarafin, Ramon Durazo-Arvizu, Lu Tian, Karen W. Phinney, Susan Tai, Johanna Camara, Hubert W. Vesper, Joyce M. Merkel, Evan Green, Christopher T. Sempos, Stephen P. Brooks
Background: The Canadian Health Measures survey (CHMS) is an ongoing cross sectional national survey that includes a measure of 25-hydroxyvitamin D (25(OH)D) by Diasorin LIAISON immunoassay. The survey repeats approximately every two years with a new

Influence of Chemical Straightening on the Stability of Drugs of Abuse in Hair

October 8, 2014
Author(s)
Jeanita Pritchett, Karen W. Phinney
Chemical straightening, also known as a relaxer, is ubiquitously used among African American women to obtain straighter hair compared with their natural tresses. This study focused on the stability of drugs of abuse in hair after a single application of

Multiplexed LC-MS/MS Assay for Urine Albumin

July 24, 2014
Author(s)
Ashley B. Green, David M. Bunk, Karen W. Phinney
Targeted Multiplexed Assay for Urinary Albumin Quantification Ashley Beasley-Green, Nijah M. Burris, David Bunk, Karen Phinney Biomolecular Measurement Division National Institute of Standards and Technology, Gaithersburg, MD ABSTRACT Urinary excretion of

Quantitative bottom-up proteomics depends on digestion conditions

December 2, 2013
Author(s)
Mark S. Lowenthal, Yuxue Liang, Karen W. Phinney, Stephen E. Stein
Accurate quantification is a fundamental requirement in the fields of proteomics and biomarker discovery, and for clinical diagnostic assays. To demonstrate the extent of quantitative variability in measurable peptide concentrations due to differences

Development of a Standard Reference Material for Metabolomics Research

November 4, 2013
Author(s)
Karen W. Phinney, Guillaume Ballihaut, Mary Bedner, Johanna Camara, Steven J. Christopher, William C. Davis, Nathan G. Dodder, Brian E. Lang, Stephen E. Long, Mark S. Lowenthal, Elizabeth A. McGaw, Karen E. Murphy, Bryant C. Nelson, Jocelyn L. Prendergast, Jessica L. Reiner, Catherine A. Rimmer, Lane C. Sander, Michele M. Schantz, Katherine E. Sharpless, Lorna T. Sniegoski, Susan S. Tai, Jeanice M. Brown Thomas, Thomas W. Vetter, Michael J. Welch, Stephen A. Wise, Laura J. Wood, William F. Guthrie, Robert C. Hagwood, Stefan D. Leigh, James H. Yen, Nien F. Zhang, Madhu Chaurhary-Webb, Huiping Chen, Bridgette Haynes, Donna J. LaVoie, Leslie F. McCoy, Shahzad S. Momin, Neelima Paladugula, Elizabeth C. Pendergrast, Christine M. Pfeiffer, Carissa D. Powers, Zia Fazili-Qari, Daniel Rabinowitz, Michael E. Rybak, Rosemary L. Schleicher, Mary Xu, Mindy Zhang, Arthur L. Castle, Brandi S. Benford, Gauthier Eppe

Metabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950) GC/MS, LC/MS, NMR and Clinical Laboratory Analyses, Libraries and Web-based resources

October 22, 2013
Author(s)
Yamil Simon, Mark S. Lowenthal, Lisa E. Kilpatrick, Maureen L. Sampson, Kelly H. Telu, Paul A. Rudnick, William G. Mallard, Daniel W. Bearden, Tracey B. Schock, Dmitrii V. Tchekhovskoi, Niksa Blonder, Xinjian Yan, Yuxue Liang, Yufang Zheng, William E. Wallace, Pedatsur Neta, Karen W. Phinney, Alan T. Remaley, Stephen E. Stein
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profiling, i.e., semi-quantitative monitoring of all chemical constituents of biological fluids. In this work, we have

The Development and Implementation of Quality Assurance Programs to Support Nutritional Measurements

May 24, 2013
Author(s)
Lane C. Sander, Mary Bedner, David L. Duewer, Katrice A. Lippa, Melissa M. Phillips, Karen W. Phinney, Catherine A. Rimmer, Michele M. Schantz, Katherine E. Sharpless, Susan Tai, Jeanice M. Brown Thomas, Stephen A. Wise, Laura J. Wood, J. M. Betz, Paul M. Coates
The National Institute of Standards and Technology administers quality assurance programs devoted to improving measurements of nutrients and related metabolites in foods, dietary supplements, and serum and plasma samples. These programs have been developed

A reference system for urinary albumin: current status

May 1, 2013
Author(s)
David M. Bunk, Karen W. Phinney, John C. Lieske, Olga Bondar, W. Greg Miller, Lorin M. Bachmann, Andrew S. Narva, Yoshihisa Itoh, Schimmel Heinz
Background: Increased urinary excretion of albumin reflects kidney damage and is a recognized risk factor for progression of renal and cardiovascular disease. Considerable inter-method differences have been reported for both albumin and creatinine
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