QconCAT: Internal Standard for Protein Quantification
Kerry M. Bauer, Karen W. Phinney, Illarion V. Turko
Protein quantification based on stable-isotope labeling mass spectrometry (SIL-MS) involves adding known quantities of stable-isotope labeled internal standards into biological samples. The internal standards are analogous to analyte molecules and quantification is achieved by comparing signals from isotope-labeled and analyte molecules. This methodology is broadly applicable to proteomics research, biomarker discovery and validation, and clinical studies; which require accurate and precise protein abundance measurements. One such internal standard platform for protein quantification are concatenated peptides (QconCAT). This paper describes a protocol for the design, expression, characterization, and application of the QconCAT strategy for protein quantification.
QconCAT, stable isotope labeling, mass spectrometry, proteomics, protein quantification, protocol
, Phinney, K.
and Turko, I.
QconCAT: Internal Standard for Protein Quantification, Isotope labeling of biomolecules Part A & B, Methods in Enzymology (Elsevier), Oxford, -1, [online], https://doi.org/10.1016/bs.mie.2015.09.022
(Accessed November 29, 2023)