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Search Publications by

John J. Kasianowicz (Fed)

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Displaying 1 - 25 of 80

Anthrax Toxin Functional Assay: Lethal Factor Binding to Protective Antigen Ion Channel

October 12, 2021
Kelly Halverson, R G. Panchal, T L. Nguyen, S Little, Martin Misakian, Sina Bavari, John J. Kasianowicz
Bacillus anthracis secretes three toxins (protective antigen, PA; lethal factor, LF; and edema factor, EF) that are the molecular basis of anthrax-induced cell death. We studied the functional interaction between LF and activated PA (PA63) in planar

Molecular-scale Structural and Functional Characterization of Sparsely Tethered Bilayer Membranes

October 12, 2021
Duncan McGillivray, Gintaras Valincius, David J. Vanderah, W Febo-Ayala, John T. Woodward IV, F Hienrich, John J. Kasianowicz, M Losche
Surface-tethered biomimetic bilayer membranes (tethered lipid bilayer membranes, tBLMs) have been formed on gold surfaces from phospholipids and a synthetic 1-thiahexa(ethy lene oxide) lipid, WC14. They have been characterized using electrochemical

The Mitochondrial Apoptosis-Induced Channel MAC and Bax Channels Are Regulated by Cytochrome C

October 12, 2021
Li Guo, D Pietkiewicz, M M. Pavlov, John J. Kasianowicz, L M. Dejean, S J. Korsmeyer, B Antonsson, K W. Kinnally
l-2 family proteins regulate the appearance of the high-conductance channel MAC (mitochondrial apoptosis-induced channel) 1 in the outer membranes of mitochondria early in apoptosis and regulate the release of cytochrome c during commitment to apoptosis

Ultraviolet Radiation Technologies and Healthcare Associated Infections: Standards and Metrology Needs

August 20, 2021
Dianne L. Poster, C Cameron Miller, Richard Martinello, Norman Horn, Michael T. Postek, Troy Cowan, Yaw S. Obeng, John J. Kasianowicz
The National Institute of Standards and Technology (NIST) hosted an international workshop on ultraviolet-C (UV-C) disinfection technologies on January 14 – 15, 2020 in Gaithersburg, Maryland in collaboration with the International Ultraviolet Association

A Comparison of Ion Channel Current Blockades Caused by Individual Poly(ethylene glycol) Molecules and Polyoxometalate Nanoclusters

June 28, 2019
Haiyan Wang, John J. Kasianowicz, Joseph W. Robertson, Dianne L. Poster, Jessica Ettedgui
Proteinaceous nanometer-scale pores have been used to detect and physically characterize many different types of molecules at the single molecule limit. The method is based on the ability to measure the transient reduction in the ionic channel conductance

High Resolution Physical Characterization of Single Metallic Nanoparticles

June 28, 2019
Jessica Ettedgui, Jacob Forstater, Joseph W. Robertson, John J. Kasianowicz
Discrete metal oxygen clusters, polyoxometalates (POMs), can be detected at the single molecule limit, using a biological nanopore-based electronic platform. The method provides a complementary approach to traditional analytical chemistry tools utilized in

Determining the physical properties of molecules with nanometer-scale pores

January 30, 2018
Haiyan Wang, Jessica Ettedgui, Jacob Fortater, Joseph W. Robertson, Joseph Reiner, Huisheng Zhang, Siping Chen, John J. Kasianowicz
Nanometer-scale pores have recently been developed for the detection, characterization, and quantitation of a wide range of analytes (e.g., ions, polymers, proteins, anthrax toxins, neurotransmitters, and synthetic nanoparticles), and for DNA sequencing

Novel Nanofluidic Chemical Cells based on Self-Assembled Solid-State SiO2 Nanotubes

August 30, 2017
Hao Zhu, Haitao Li, Arvind Balijepalli, Joseph W. Robertson, Sergiy Krylyuk, Albert Davydov, John J. Kasianowicz, John S. Suehle, Qiliang Li
Novel nanofluidic chemical cells based on self-assembled solid-state SiO2 nanotubes on silicon- on-insulator (SOI) substrates have been successfully fabricated and tested. The vertical SiO2 nanotubes with a smooth cavity are built from Si nanowires which

MOSAIC: A Modular Single Molecular Analysis Interface for Decoding Multi-state Nanopore Data

October 31, 2016
Jacob H. Forstater, Kyle Briggs, Joseph W. Robertson, Jessica H. Benjamini, Olivier M. Marie-Rose, Canute I. Vaz, John J. Kasianowicz, Vincent Tabard-Cossa, Arvind Balijepalli
Single molecule measurements with nanometer-scale pores are being developed for a wide range of diagnostic and therapeutic applications.  Critical to the method is the ability to accurately and precisely detect the presence of an analyte, quantify its

Real-Time, Single Molecule Electronic DNA Sequencing by Synthesis Using Polymer Tagged Nucleotides on a Nanopore Array

May 10, 2016
Carl Fuller, Shiv Kumar, Mintu Porel, Michen Chien, Arek Bibillo, P. B. Stranges, Michael Dorwart, Chuanjua Tao, Zengmin Li, Wenjing Guo, Shundi Shi, Daniel Korenblum, Andrew Trans, Anne Aguirre, Edward Liu, Takeshi Harada, James Pollard, Ashwini Bhat, Cynthia Cech, Alexander Yang, Cleoma Spilman, Mirko Palla, Jennifer Hovis, Roger Chen, Irina Morozova, Sergey Kalachikov, James Russo, John J. Kasianowicz, Stefan Roever, George Church, Jingyue Ju
DNA sequencing by synthesis (SBS) offers a robust platform to decipher nucleic acid sequences. Recently, we developed a single molecule nanopore-based SBS strategy (NanoSBS) that accurately distinguishes four bases by electronically detecting and

Proximal Capture Dynamics for a Single Biological Nanopore Sensor

June 23, 2015
John J. Kasianowicz
Single nanopore sensors enable capture and analysis of molecules that are driven to the pore entry from bulk solution. However, the distance between an analyte and the nanopore opening limits the detection efficiency. A theoretical basis for predicting

Quantifying short-lived events in multistate ionic current measurements

January 7, 2014
Arvind K. Balijepalli, Jessica H. Benjamini, Andrew T. Cornio, Joseph W. Robertson, Kin P. Cheung, John J. Kasianowicz, Canute I. Vaz
We developed a generalized technique to characterize polymer!nanopore interactions via single channel ionic current measurements. Physical interactions between analytes, such as DNA, proteins, or synthetic polymers, and a nanopore cause multiple discrete

Temperature sculpting in yoctoliter volumes

February 14, 2013
Joseph E. Reiner, Joseph W. Robertson, Daniel L. Burden, Lisa K. Burden, Arvind Balijepalli, John J. Kasianowicz
The ability to perturb large ensembles of molecules from equilibrium led to major advances in understanding reaction mechanisms in chemistry and biology. Here, we demonstrate the ability to control, measure, and make use of rapid temperature changes of

The Effects of Diffusion on an Exonuclease/Nanopore-Based DNA Sequencing Engine

December 7, 2012
Joseph E. Reiner, Joseph W. Robertson, Arvind Balijepalli, Daniel L. Burden, Bryon S. Drown, John J. Kasianowicz
The ability to electronically detect and characterize individual polynucleotides as they are driven through a single protein ion channel may eventually prove useful for rapidly sequencing DNA (base-by-base) in a ticker tape-like fashion. More recently, a

PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis

September 21, 2012
Shiv Kumar, Tao Chuanjuan, Chien Minchen, Hellner Brittney, Joseph W. Robertson, Arvind Balijepalli, Li Zengmin, James J. Russo, Joseph E. Reiner, John J. Kasianowicz, Jingyue Ju
There is a significant need to accurately sequence single DNA and RNA molecules for personalized medicine. We describe a novel nanopore-based sequencing by synthesis (SBS) strategy that will accurately differentiate at single molecule level four different