Solution Analysis by Threading Single Molecules Through a Self-Assembled Nanometer-Scale Pore
John J. Kasianowicz, S. E. Henrickson, B Robertson, H H. Weetall
We show here the principles for using a single nanometer-scale pore to detect a wide variety of analytes (e.g. small ions, proteins, antigens, antibodies, DNA sequences). The analyte recognition site or hapten is covalently attached to a linear polymer that threads through the pore. As predicted, analyte binding to the polymer changes how the latter interacts with a pore in two mutually exclusive ways. We discuss how this method can be extended for the simultaneous, real-time detection of a multiple analytes. In addition, the region inside the model pore (a-hemolysin) that commits DNA to transport through the channel was deduced using *molecular rulers* comprised of an analyte-DNA complex.
alpha-hemolysin, analyte, biosensor, DNA, ion channel, polymer
, Henrickson, S.
, Robertson, B.
and Weetall, H.
Solution Analysis by Threading Single Molecules Through a Self-Assembled Nanometer-Scale Pore, Science
(Accessed January 16, 2022)