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Solution Analysis by Threading Single Molecules Through a Self-Assembled Nanometer-Scale Pore

Published

Author(s)

John J. Kasianowicz, S. E. Henrickson, B Robertson, H H. Weetall

Abstract

We show here the principles for using a single nanometer-scale pore to detect a wide variety of analytes (e.g. small ions, proteins, antigens, antibodies, DNA sequences). The analyte recognition site or hapten is covalently attached to a linear polymer that threads through the pore. As predicted, analyte binding to the polymer changes how the latter interacts with a pore in two mutually exclusive ways. We discuss how this method can be extended for the simultaneous, real-time detection of a multiple analytes. In addition, the region inside the model pore (a-hemolysin) that commits DNA to transport through the channel was deduced using *molecular rulers* comprised of an analyte-DNA complex.
Citation
Science

Keywords

alpha-hemolysin, analyte, biosensor, DNA, ion channel, polymer

Citation

Kasianowicz, J. , Henrickson, S. , Robertson, B. and Weetall, H. (2021), Solution Analysis by Threading Single Molecules Through a Self-Assembled Nanometer-Scale Pore, Science (Accessed January 16, 2022)
Created October 12, 2021