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Displaying 26 - 50 of 164

Real-Time, Single Molecule Electronic DNA Sequencing by Synthesis Using Polymer Tagged Nucleotides on a Nanopore Array

May 10, 2016
Author(s)
Carl Fuller, Shiv Kumar, Mintu Porel, Michen Chien, Arek Bibillo, P. B. Stranges, Michael Dorwart, Chuanjua Tao, Zengmin Li, Wenjing Guo, Shundi Shi, Daniel Korenblum, Andrew Trans, Anne Aguirre, Edward Liu, Takeshi Harada, James Pollard, Ashwini Bhat, Cynthia Cech, Alexander Yang, Cleoma Spilman, Mirko Palla, Jennifer Hovis, Roger Chen, Irina Morozova, Sergey Kalachikov, James Russo, John J. Kasianowicz, Stefan Roever, George Church, Jingyue Ju
DNA sequencing by synthesis (SBS) offers a robust platform to decipher nucleic acid sequences. Recently, we developed a single molecule nanopore-based SBS strategy (NanoSBS) that accurately distinguishes four bases by electronically detecting and

Proximal Capture Dynamics for a Single Biological Nanopore Sensor

June 23, 2015
Author(s)
John J. Kasianowicz
Single nanopore sensors enable capture and analysis of molecules that are driven to the pore entry from bulk solution. However, the distance between an analyte and the nanopore opening limits the detection efficiency. A theoretical basis for predicting

Quantifying short-lived events in multistate ionic current measurements

January 7, 2014
Author(s)
Arvind K. Balijepalli, Jessica H. Benjamini, Andrew T. Cornio, Joseph W. Robertson, Kin P. Cheung, John J. Kasianowicz, Canute I. Vaz
We developed a generalized technique to characterize polymer!nanopore interactions via single channel ionic current measurements. Physical interactions between analytes, such as DNA, proteins, or synthetic polymers, and a nanopore cause multiple discrete

Temperature sculpting in yoctoliter volumes

February 14, 2013
Author(s)
Joseph E. Reiner, Joseph W. Robertson, Daniel L. Burden, Lisa K. Burden, Arvind Balijepalli, John J. Kasianowicz
The ability to perturb large ensembles of molecules from equilibrium led to major advances in understanding reaction mechanisms in chemistry and biology. Here, we demonstrate the ability to control, measure, and make use of rapid temperature changes of

The Effects of Diffusion on an Exonuclease/Nanopore-Based DNA Sequencing Engine

December 7, 2012
Author(s)
Joseph E. Reiner, Joseph W. Robertson, Arvind Balijepalli, Daniel L. Burden, Bryon S. Drown, John J. Kasianowicz
The ability to electronically detect and characterize individual polynucleotides as they are driven through a single protein ion channel may eventually prove useful for rapidly sequencing DNA (base-by-base) in a ticker tape-like fashion. More recently, a
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