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Search Publications by: Michael J. Tarlov (Fed)

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Displaying 1 - 25 of 61

Interlaboratory Studies using the NISTmAb to Advance Biopharmaceutical Structural Analytics

May 5, 2022
Katharina Yandrofski, Trina Mouchahoir, M. Lorna De Leoz, David L. Duewer, Jeffrey W. Hudgens, Kyle Anderson, Luke Arbogast, Frank Delaglio, Robert Brinson, John Marino, Karen W. Phinney, Michael J. Tarlov, John E. Schiel
Biopharmaceuticals such as monoclonal antibodies are required to be rigorously characterized using a wide range of analytical methods. Various material properties must be characterized and well controlled to assure that clinically relevant features and

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays

October 12, 2021
Gloria Thomas, Emad E. El-Giar, Laurie E. Locascio, Michael J. Tarlov
The integration of immunoassays in microfluidic devices is a rapidly developing research area combining the power of immunoassays with the inherent benefits of microfluidics. Here, a general overview of microfluidic-based immunoassays is presented along

Quantitative Surface Characterization of DNA Immobilized on Gold

October 12, 2021
D Y. Petrovykh, H Y. Kimura-Suda, L J. Whitman, Michael J. Tarlov
We describe the complementary use of x-ray photoelectron spectroscopy (XPS) and fourier transform infrared (FTIR) spectroscopy to quantitatively characterize the immobilization of thiolated poly(dT025 single-stranded DNA on gold. When electron attenuation

A Lectin-Based Gold Nanoparticle Assay for Probing Glycosylation of Glycoproteins

April 17, 2012
Germarie Sanchez-Pomales, Todd A. Morris, James Falabella, Michael J. Tarlov, Rebecca A. Zangmeister
We report a glycoanalysis method in which lectins are used to probe the glycans of glycoproteins that are adsorbed on gold nanoparticles. A model mannose-presenting glycoprotein, ribonuclease B (RNase B), and the therapeutic monoclonal antibody (mAb)

Electrospray – Differential Mobility Analysis as an Orthogonal Tool to Size Exclusion Chromatography for Characterization of Protein Aggregates

March 12, 2012
Suvajyoti S. Guha, Joshua R. Wayment, Michael J. Tarlov, Michael R. Zachariah
The biopharmaceutical industry characterizes and quantifies aggregation of protein therapeutics using multiple analytical techniques to cross validate results. Here we demonstrate the use of electrospray-differential mobility analysis (ES-DMA), a gas-phase

Method for Determining the Absolute Number Concentration of Nanoparticles from Electrospray

October 27, 2011
Mingdong M. Li, Suvajyoti S. Guha, Rebecca A. Zangmeister, Michael J. Tarlov, Michael R. Zachariah
We have developed a simple, fast, and accurate method to measure the absolute number concentration of nanoparticles in solution. The method combines electrospray-differential mobility analysis (ES-DMA) with a statistical analysis of droplet induced

Quantification and Compensation of Unintentional Analyte Aggregation in Electrospray Sampling

March 30, 2011
Mingdong M. Li, Suvajyoti S. Guha, Rebecca A. Zangmeister, Michael J. Tarlov, Michael R. Zachariah
Electrospray (ES) sources are commonly used to introduce non-volatile materials (e.g. nanoparticles, proteins, etc.) in to the gas phase for characterization by mass spectrometry and ion mobility. Recent studies in our group using electrospray ion mobility

Characterization of gold nanoparticles modified with single-stranded DNA using analytical ultracentrifugation and dynamic light scattering

August 3, 2010
James Falabella, Tae Joon Cho, Dean C. Ripple, Vincent A. Hackley, Michael J. Tarlov
We report the characterization of gold nanoparticles modified with thiol-terminated single stranded DNA using analytical ultracentrifugation and dynamic light scattering. Sedimentation coefficients of nominally 10 and 20 nm diameter gold nanoparticles

Nanotube Length Distributions Measured from Suspension by Electrospray-Differential Mobility Analysis

December 4, 2009
Leonard F. Pease III, De-Hao D. Tsai, Jeffrey A. Fagan, Barry J. Bauer, Rebecca A. Zangmeister, Michael J. Tarlov, Michael R. Zachariah
We present the first systematic characterization of well dispersed, length sorted single-walled carbon nanotubes (SWCNTs) by electrospray-differential mobility analysis (ES-DMA). Although understanding geometric properties of SWCNTs including their length

Selective Binding of RNAse B Glycoforms by Polydopamine-immobilized Concanavalin A

June 10, 2009
Todd A. Morris, Alexander W. Peterson, Michael J. Tarlov
Glycoanalysis is important in the manufacture and quality control of protein therapeutics. An emerging method for glycoanalysis is the use of lectin arrays. Critical to the performance of these arrays is the immobilization of the lectin molecules

A Method for Fabricating Conductometric Gas Sensors of Metal Oxide Nanotubes Formed in Porous Aluminum Oxide Membranes

February 2, 2009
Reit Artzi-Gerlitz, Kurt D. Benkstein, David L. Lahr, Joshua L. Hertz, Christopher B. Montgomery, John E. Bonevich, Stephen Semancik, Michael J. Tarlov
We describe a general, simple strategy for fabricating conductometric gas sensors from metal oxide nanotubes formed in nanoporous aluminum oxide membranes. In this approach, the aluminum oxide membrane serves as both a template for growth of the sensing

Quantifying the Surface Coverage of Conjugate Moleucles on Functionalized Nanoparticles

November 22, 2007
Leonard F. Pease III, De-Hao D. Tsai, Rebecca A. Zangmeister, M Zachariah, Michael J. Tarlov
Electrospray-differential mobility analysis (ES-DMA) represents an attractive tool to determine directly the coverage and conformation of long, flexible molecules on engineered nanoparticles. For example, nanoparticles coated with single-stranded DNA

Synthesis and Structural Characterization of Glucopyranosylamide Films on Gold

January 16, 2007
Mridula Kadalbajoo, J J. Park, A Opdahl, Hiromi Suda, Kitchens A. C, Jayne C. Garno, J Batteas, Michael J. Tarlov, Phillip DeShong
Stereoselective synthesis of a- and b-linked glucopyranosylamide derivatives via the in situ generation of glucosyl isoxazolines has been performed utilizing a modified Staudinger methodology. Glucopyranosylamides were derivatized with thiol

Electrochemical Study of Chitosan Films Deposited on Gold Electrodes

July 28, 2006
Rebecca A. Zangmeister, J J. Park, G W. Rubloff, Michael J. Tarlov
We report the electrochemical characterization of chitosan films deposited on gold electrodes. Cyclic voltammetry was used to characterize the deposition and electroactivity of chitosan coated gold electrodes. Chitosan films were found to deposit at a gold

Alkanethiols on Platinum: Multicomponent Self-Assembled Monolayers

March 14, 2006
D Y. Petrovykh, H Y. Kimura-Suda, A Opdahl, Lee J. Richter, Michael J. Tarlov, L J. Whitman
We have studied the formation of self-assembled monolayers (SAMs) of n-alkanethiols on platinum thin films using X-ray photoelectron spectroscopy (XPS), reflection-absorption infrared spectroscopy (RAIRS), spectroscopic ellipsometry (SE), and contact angle

Nucleobase Orientation and Ordering in Films of Single-Stranded DNA on Gold

January 11, 2006
D Y. Petrovykh, V Perez-Dieste, A Opdahl, H Y. Kimura-Suda, J M. Sullivan, Michael J. Tarlov, F J. Himpsel, L J. Whitman
The structure of single-stranded DNA (ssDNA) immobilized on surfaces is critical to applications exploiting the molecular recognition function of nucleic acids.1-3 Although traditional surface analysis can be applied to adsorbed biomolecules,1 the