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Search Publications

NIST Authors in Bold

Displaying 326 - 350 of 1275

Best practices for benchmarking germline small-variant calls in human genomes

March 11, 2019
Author(s)
Justin M. Zook, Marc L. Salit
Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant- calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part

Engineering ClpS for Selective and Enhanced N-terminal Amino Acid Binding

March 1, 2019
Author(s)
Jennifer A. Tullman, Zvi Kelman, John P. Marino
One of the central challenges in the development of single-molecule protein sequencing technologies is achieving high-fidelity, sequential recognition and detection of specific amino acids that comprise the peptide sequence. An approach towards achieving

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

February 20, 2019
Author(s)
Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system

Proceedings of the First Workshop on Standards for Microfluidics

January 28, 2019
Author(s)
Darwin R. Reyes-Hernandez, Henne van Heeren
In the last two decades, the microfluidics/lab-on-a-chip field has evolved from the concept of micro total analysis systems, where systems with integrated pretreatment and analysis of chemicals were envisioned, to what is known today as lab-on-a-chip

CrowdVariant: a crowdsourcing approach to curate copy number variants

January 6, 2019
Author(s)
Justin M. Zook, Marc L. Salit, Peyton Greenside, Ryan Poplin, Mark DePristo, Madeleine Cule
Copy number variants (CNVs) are an important type of genetic variation and play a causal role in many diseases. However, they are also notoriously difficult to identify accurately from next-generation sequencing (NGS) data. For larger CNVs, genotyping

Cooperative Dynamics in a Model DPPC Membrane Arise from Membrane Layer Interactions

December 19, 2018
Author(s)
Kiley Kennedy, Neha Shafique, Jack F. Douglas, Francis W. Starr
The dynamics of model membranes can be highly heterogeneous, especially in more ordered dense phases. Experimentally, monolayers are often studied as a model system to infer the properties of membranes. To better understand the origins of this

Report from the Winter Mid-Atlantic Microbiome Meet-up on Biodefense and Pathogen Detection, College Park, MD January 10th, 2018

November 5, 2018
Author(s)
Jacquelyn S. Meisel, Daniel Nasko, Brian Brubach, Victoria Cepeda Espinoza, Jessica Chopyk, Hector Corrada Bravo, Marcus Fedarko, Jay Ghurye, Kiran Javkar, Nathan D. Olson, Nidhi Shah, Sarah M. Allard, Adam L. Bazinet, Nichloas H. Bergman, Alexis Brown, Greg Caporaso, Sean Conlan, Jocelyn DiRuggiero, Samuel Forry, Nur A. Hasan, John Julias, Paul M. Luethy, Donald K. Milton, Brian Ondov, Sarah Preheim, Tara O'Toole, Stephanie Rogers, M.J. Rosovitz, Eric G. Sakowski, Nils O. Schliebs, Daniel D. Sommer, Krista Ternus, German Uritskiy, Sean X. Zhang, Mihai Pop, Todd Treangen
The Mid-Atlantic Microbiome Meet-up (M3) organization held its fourth meeting on January 10, 2018 in College Park, Maryland. M3 brings together academic, government, and industry groups in the broadly-defined mid-Atlantic region to share ideas and develop

Innovative Approaches to Combat Healthcare-Associated Infections Using Efficacy Standards Developed Through Industry and Federal Collaboration

October 5, 2018
Author(s)
Dianne L. Poster, Carl C. Miller, Yaw S. Obeng, Michael T. Postek, Troy E. Cowan, Richard A. Martinello
Nation-wide, healthcare-associated infections (HAIs) infect one in every 25 hospital patients, account for more than 100,000 deaths and increase medical costs by around $96-147B, each year. Ultraviolet-C (UV-C) antimicrobial devices are shown to reduce the

Combinatorial Cassettes for Higher-Throughput Screening of Osteogenesis

October 4, 2018
Author(s)
Carl G. Simon Jr., Subhadip Bodhak, Hariharan K. Iyer, Luis Fernandez de Castro Diaz, Pam Robey, Azusa Maeda, Sergei Kuznetsov
Animal models are the most biologically relevant method for measuring the osteogenic capability of bone graft formulations. However, animal experiments are slow, tedious and the results can be highly variable, even when conducted within the same lab. In

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Prasad T. Reddy, Robert G. Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Robert Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen, Zvi Kelman, Prasad T. Reddy
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost

Microscopic Identification of Micro-Organisms on Pre-Viking Swedish Hillfort Glass

September 30, 2018
Author(s)
Bruce Arey, Edward Vicenzi, Rolf Sjoblom, Robert Koestler, Paula DePriest, Thomas Lam, David Peeler, John McCloy, Albert Kruger, Jamie Weaver
Borborg Hillfort is a vitrified hillfort located in the Husby-Långhundra parish in Uppland Sweden (Fig. 1A). The site dates to the Great Migration Period, around 740 ± 100 CE. [1]. Two types of glasses have been identified within the ramparts – one that is

Methodology for evaluating and comparing fluorescence measurement capabilities: Multi-site study of 23 flow cytometers

September 23, 2018
Author(s)
David R. Parks, Wayne A. Moore, Ryan Brinkman, Yong Chen, Danilo Condello, Faysal E. Khettabi, John P. Nolan, Stephen P. Perfetto, Doug Redelman, Josef Spidlen, Jonathan V. Dyke, Lili Wang, James C. Wood
We developed measurement methods and analysis procedures to perform inter-instrument comparisons and used them to evaluate and compare the fluorescence measurement capabilities among 23 cytometers in 9 laboratories selected to provide a range of

Structure of the Fc Fragment from the NIST Reference Antibody RM8671

September 1, 2018
Author(s)
David Travis Gallagher, Connor V. Galvin, Ioannis Karageorgos
As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in

High-speed alignment optimization of digital optical phase conjugation systems based on autocovariance analysis in conjunction with orthonormal rectangular polynomials

August 28, 2018
Author(s)
Ashton S. Hemphill, Yuecheng Shen, Jeeseong C. Hwang, Lihong V. Wang
Digital optical phase conjugation (DOPC) enables many optical applications by permitting focusing of light through scattering media. However, DOPC systems require precise alignment of all optical components, particularly of the spatial light modulator (SLM

Conference Report: Representing Ethnic Diversity for Precision Medicine

August 23, 2018
Author(s)
Justin M. Zook, Luke Hickey, Benedict Paten, Robert Sebra, Valerie Schneider
There is a pressing need to better represent ethnic diversity with genomic resources — and to do so in a way that maximizes utility for people working with the Genome Reference Consortium’s human reference genome. That was the theme of a panel discussion
Displaying 326 - 350 of 1275
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