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Displaying 26 - 50 of 233

Low-template DNA: A single DNA analysis or two replicates?

April 18, 2016
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
This study investigates the following two questions: (i) Should the DNA analyst concentrate the DNA extract into a single amplification or should he/she split it up to do two replicates? (ii) Given the electropherogram obtained from a first analysis, is it

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 24, 2016
Sandra M. Da Silva, Lindsay Harris, Nathanael David Olson, Steven Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow
Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results

Sequence variation of 22 autosomal STR loci detected by next generation sequencing

December 1, 2015
Katherine Gettings, Kevin M. Kiesler, Seth A. Faith, Elizabeth Montano, Christine H. Baker, Brian A. Young, Richard A. Guerreri, Peter Vallone
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories

Sequence-based Analysis of Stutter at STR Loci: Characterization and Utility

September 23, 2015
Rachel A. Aponte, Katherine Gettings, David L. Duewer, Michael D. Coble, Peter Vallone
The development of next generation sequencing (NGS) technologies creates the potential for changing the method by which the forensic science community genotypes short tandem repeat (STR) loci. While the capabilities of NGS are promising, moving from

Uncertainty in the number of contributors in the proposed new CODIS set

July 17, 2015
Michael D. Coble, Jo-Anne Bright, John S. Buckleton, James Curran
The probability that multiple contributors are detected within a forensic DNA profile improves as more highly polymorphic loci are analysed. The assignment of the correct number of contributors to a profile is important when interpreting the DNA profiles

The Future of Forensic DNA Analysis

June 22, 2015
John M. Butler
The author's thoughts and opinions on where the field of forensic DNA testing is headed for the next decade are provided in the context of where the field has come over the past 30 years. Like the Olympic motto of "faster, higher, stronger", forensic DNA

Performance of a next generation sequencing SNP assay on degraded DNA

May 27, 2015
Katherine Gettings, Kevin M. Kiesler, Peter Vallone
Forensic DNA casework samples are often of insufficient quantity or quality to generate full profiles by conventional DNA typing methods. Amplification of STR loci is inherently limited in samples containing degraded DNA, as the cumulative size of repeat

Rapid PCR of STR Markers: Applications to Human Identification

April 23, 2015
Peter Vallone, Erica Romsos
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection

Rapid PCR Protocols for Forensic DNA Typing on Six Thermal Cycling Platforms

August 22, 2014
Peter Vallone, Erica Romsos
Rapid PCR protocols for the amplification of typing short tandem repeat multiplexes were evaluated on 6 different thermal cyclers. PCR primers from the commercially available multiplex short tandem repeat typing kit Identifiler were used to target 15 STR

Biology and Genetics of New Autosomal STR Loci Useful for Forensic DNA Analysis

August 19, 2013
John M. Butler, Carolyn R. Steffen
Short tandem repeats (STRs) are regions of tandemly repeated DNA segments found throughout the human genome that vary in length (through insertion, deletion, or mutation) with a core repeated DNA sequence. Forensic laboratories commonly use tetranucleotide

Haplotype Data for 23 Y-chromosome markers in four U.S. population groups

May 1, 2013
Michael D. Coble, Carolyn R. Steffen, John M. Butler
The PowerPlex Y23 kit contains 23 Y-chromosomal loci including all 17 of the markers in the Yfiler Y-STR kit plus six additional markers: DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643. We have typed 1032 unrelated population samples from four self

NIST Gold Nanoparticle Reference Materials Do Not Induce Oxidative DNA Damage

February 1, 2013
Bryant C. Nelson, Donald H. Atha, John T. Elliott, Bryce J. Marquis, Elijah J. Petersen, Danielle Cleveland, Stephanie S. Watson, I-Hsiang Tseng, Andrew Dillon, Melissa Theodore, Joany Jackman
Well-characterized, nanoparticle reference materials are urgently needed for nanomaterial toxicity studies. The National Institute of Standards and Technology has developed three gold nanoparticle (AuNP) reference materials (10 nm, 30 nm, 60 nm) to address

Oxidatively Induced DNA Damage and Cancer

December 31, 2012
M Miral Dizdar
Endogenous and exogenous sources cause oxidatively induced DNA damage in living organisms by a variety of mechanisms. Resulting DNA lesions are mutagenic and, unless repaired, lead to a variety of mutations and consequently to genetic instability, which a
Displaying 26 - 50 of 233