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Displaying 26 - 50 of 241

DNA Error Rates

December 7, 2016
John Butler
The 1993 U.S. Supreme Court ruling in Daubert vs. Merrell-Dow Pharmaceuticals insists on knowledge regarding error rates and their application when evidence is introduced into court [1]. The 1996 National Research Council (NRC II) report The Evaluation of

DNA Commission of the International Society for Forensic Genetics: Recommendations on the validation of software programs performing biostatistical calculations for forensic genetics applications

September 4, 2016
Michael D. Coble, John S. Buckleton, John M. Butler, Barbara Guttman
The use of biostatistical software programs to assist in data interpretation and calculate likelihood ratios is essential to forensic geneticists and part of the daily case work flow for both kinship and DNA identification laboratories. Previous

Evaluation of Forensic DNA Mixture Evidence: Guidelines for Evaluation, Interpretation, and Statistical Calculations using the Combined Probability of Inclusion

August 31, 2016
Frederick R. Bieber, John Buckleton, Bruce Budowle, John Butler, Michael D. Coble
The evaluation and interpretation of forensic DNA mixture evidence faces greater interpretational challenges due to increasingly complex mixture evidence. Such challenges include: casework involving low quantity or degraded evidence leading to allele and

Is the Factor of 10 Still Applicable Today?

July 13, 2016
Simone N. Gittelson, John S. Buckleton
The assignment of the weight of DNA evidence depends on a number of factors (allele probability estimates, the population genetic model used, the value of the coancestry coefficient, etc.). One of these factors is the allele probability estimates from a

Expected net gain data of low-template DNA analyses

May 30, 2016
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
Low-template DNA analyses are affected by stochastic effects which can produce a configuration of peaks in the electropherogram (EPG) that is different from the genotype of the DNA's donor. A probabilistic and decision-theoretic model can quantify the

Low-template DNA: A single DNA analysis or two replicates?

April 18, 2016
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
This study investigates the following two questions: (i) Should the DNA analyst concentrate the DNA extract into a single amplification or should he/she split it up to do two replicates? (ii) Given the electropherogram obtained from a first analysis, is it

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 24, 2016
Sandra M. Da Silva, Lindsay Harris, Nathanael David Olson, Steven Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow
Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results

Sequence variation of 22 autosomal STR loci detected by next generation sequencing

December 1, 2015
Katherine Gettings, Kevin M. Kiesler, Seth A. Faith, Elizabeth Montano, Christine H. Baker, Brian A. Young, Richard A. Guerreri, Peter Vallone
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories

Sequence-based Analysis of Stutter at STR Loci: Characterization and Utility

September 23, 2015
Rachel A. Aponte, Katherine Gettings, David L. Duewer, Michael D. Coble, Peter Vallone
The development of next generation sequencing (NGS) technologies creates the potential for changing the method by which the forensic science community genotypes short tandem repeat (STR) loci. While the capabilities of NGS are promising, moving from

Uncertainty in the number of contributors in the proposed new CODIS set

July 17, 2015
Michael D. Coble, Jo-Anne Bright, John S. Buckleton, James Curran
The probability that multiple contributors are detected within a forensic DNA profile improves as more highly polymorphic loci are analysed. The assignment of the correct number of contributors to a profile is important when interpreting the DNA profiles

The Future of Forensic DNA Analysis

June 22, 2015
John M. Butler
The author's thoughts and opinions on where the field of forensic DNA testing is headed for the next decade are provided in the context of where the field has come over the past 30 years. Like the Olympic motto of "faster, higher, stronger", forensic DNA

Performance of a next generation sequencing SNP assay on degraded DNA

May 27, 2015
Katherine Gettings, Kevin M. Kiesler, Peter Vallone
Forensic DNA casework samples are often of insufficient quantity or quality to generate full profiles by conventional DNA typing methods. Amplification of STR loci is inherently limited in samples containing degraded DNA, as the cumulative size of repeat

Rapid PCR of STR Markers: Applications to Human Identification

April 23, 2015
Peter Vallone, Erica Romsos
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection

Rapid PCR Protocols for Forensic DNA Typing on Six Thermal Cycling Platforms

August 22, 2014
Peter Vallone, Erica Romsos
Rapid PCR protocols for the amplification of typing short tandem repeat multiplexes were evaluated on 6 different thermal cyclers. PCR primers from the commercially available multiplex short tandem repeat typing kit Identifiler were used to target 15 STR

Biology and Genetics of New Autosomal STR Loci Useful for Forensic DNA Analysis

August 19, 2013
John M. Butler, Carolyn R. Steffen
Short tandem repeats (STRs) are regions of tandemly repeated DNA segments found throughout the human genome that vary in length (through insertion, deletion, or mutation) with a core repeated DNA sequence. Forensic laboratories commonly use tetranucleotide
Displaying 26 - 50 of 241