Low-template DNA analyses are affected by stochastic effects which can produce a configuration of peaks in the electropherogram (EPG) that is different from the genotype of the DNA's donor. A probabilistic and decision-theoretic model can quantify the expected net gain (ENG) of performing a DNA analysis by the difference between the expected value of information (EVOI) and the cost of performing the analysis. This article presents data on the ENG of performing DNA analyses of low-template DNA for a single amplification, two replicate amplifications, and for a second replicate amplification given the result of a first analysis. The data were obtained using amplification kits AmpFlSTR Identifiler Plus and Promega's PowerPlex 16 HS, an ABI 3130xl genetic sequencer, and Applied Biosystem's GeneMapper ID-X software. These data are supplementary to an original research article investigating whether a forensic DNA analyst should perform a single DNA analysis or two replicate analyses from a decision-theoretic point of view, entitled Low-template DNA: a single DNA analysis or two replicates? (Gittelson et al.,2016).
Expected net gain data of low-template DNA analyses
Forensic science, LT-DNA, Replicates