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Displaying 351 - 375 of 378

Radiation-Based Quantitative Bioimaging at NIST

August 1, 2009
Author(s)
Lisa R. Karam
The National Institute of Standards and Technology (NIST) provides the physical measurements and standards in a variety of fields, including for ionizing radiation, for the US. Building on a long history of providing standards for medical x rays and

Quantitative Characterization of Quantum Dot-labeled Lambda Phage for Escherichia coli Detection

July 24, 2009
Author(s)
Peter Yim, Matthew L. Clarke, Michael McKinstry, Silvia H. De Paoli Lacerda, Leonard F. Pease III, Marina A. Dobrovolskaia, Hyeong G. Kang, David T. Read, Shanmuga Sozhamannan, Jeeseong C. Hwang
We rapidly and quantitatively detect biological pathogens by combining the specificity of bacteriophage lambda with the sensitivity of CdSe/ZnS quantum dot (QD)-based fluorescence. Specifically, QD-decorated lambda phages bind to and allow detection of

Direct Measurement of Association and Dissociation Rates of DNA Binding in Live Cells by Fluorescence Correlation Spectroscopy

July 1, 2009
Author(s)
Ariel Michelman-Ribeiro, Davide Mazza, Tilman Rosales, Timothy J. Stasevich, Boukari Hacene, Vikas Rishi, Charles Vinson, Jay R. Knutson, James G. NcNally
Measurement of live-cell binding interactions is vital for understanding the biochemical reactions that drive cellular processes. Here we develop, characterize and apply a new procedure to extract information about binding to an immobile substrate from FCS

Primary Standards for Brachytherapy Sources

June 1, 2009
Author(s)
Michael G. Mitch, Christopher G. Soares
The AAPM dosimetry protocol for LDR brachytherapy seeds that emit x-rays of energies ≤ 50 keV is described in the TG-43U1 report (Rivard et al. 2004). In this protocol, it is specified that the strength of such sources be expressed in terms of air-kerma

Dipolar Chains formed by synthesized cobalt nanocubes

May 11, 2009
Author(s)
Guangjun Cheng, Robert D. Shull, Angela R. Hight Walker
Cobalt nanocubes with an average edge length of 50 nm and the epsilon crystalline structure were synthesized via thermo-decomposition in 1,2-dichlorobenzene at 120 oC in the presence of the surfactants. These nanocubes form dipolar chains upon drying in

Terahertz spectroscopy of aqueous L-Proline in reverse micelles

November 21, 2008
Author(s)
Catherine C. Cooksey, Edwin J. Heilweil, Benjamin Greer
A new method for obtaining room temperature terahertz (THz) absorption spectra of aqueous-phase biomolecules in the frequency range 1 THz to 21 THz (35 cm-1 to 700 cm-1) is reported. The spectrum for L-Proline was acquired by solvating the amino acid

THz Investigations of Condensed Phase Biomolecular Systems

October 8, 2008
Author(s)
Kevin O. Douglass, Hailiang Zhang, Susan K. Gregurick, Karen M. Siegrist, David F. Plusquellic
Terahertz (THz) spectroscopic investigations of crystalline dipeptide nanotubes are discussed in the frequency region from 0.6 THz (2 cm-1) to 3 THz (100 cm-1). The THz region provides access to collective modes of biomolecular systems and is therefore

Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes

May 25, 2007
Author(s)
Dustin Levy, Kimberly Briggman
The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder

Characterization and Control of Lipid Layer Fluidity in Hybrid Bilayer Membranes

January 31, 2007
Author(s)
Neil A. Anderson, Lee J. Richter, John C. Stephenson, Kimberly A. Briggman
The main gel-to-liquid-crystal (LC) phase transition temperature, Tm, of the distal lipid layer in hybrid bilayer membranes (HBMs) under water was investigated using vibrational sum frequency spectroscopy (VSFS). VSFS has unique sensitivity to order

Determination of Lipid Phase Transition Temperatures in Hybrid Bilayer Membranes

September 9, 2006
Author(s)
Neil A. Anderson, Lee J. Richter, John C. Stephenson, Kimberly A. Briggman
The main gel to liquid crystal (LC) phase transition temperature, Tm, of the lipid monolayers in hybrid bilayer membranes (HBMs) was investigated using vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to probe order/disorder
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