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Eun Jin Lee, Peter Lialios, Micaila Curtis, James Williams IV, Yoontae Kim, Paul Salipante, Steven Hudson, Mandy Esch, moshe levi, Joanna Kitlinska, Stella Alimperti
Glucocorticoids (GCs) are standard-of-care treatments for inflammatory and immune disorders, and their long-term use increases the risk of osteoporosis. Although GCs decrease bone functionality, their role in bone microvasculature is incompletely
Public, reference data are one of the most important foundational resources in the modern life and biomedical sciences. Over the last 40 years, the public release and availability of genomic and macromolecule structural databases accelerated discovery
Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step toward the
Susan Debad, David Allen, Maria Teresa Baltazar, Omari Bandele, Michaela Blaylock, Paul Brown, Maureen Bunger, Julia Co, Lynn Crosby, Amber Daniel, Steven Ferguson, Kevin Ford, Goncalo Gamboa da Costa, Kristin Gilchrist, Matthew Grogg, Maureen Gwinn, Thomas Hartung, Simon Hogan, Ye Jeong, George Kass, Elaina Kenyon, Nicole Kleinstreuer, Ville Kujala, Jaehyun Lim, Patrik Lundquist, Joanna Matheson, Shaun McCullough, Angela Melton-Celsa, Steven Musser, Ilung Oh, Oluwakemi Oyetade, Sarita Patil, Elijah Petersen, Nakissa Sadrieh, Christie Sayes, Benjamin Scruggs, Yu-Mei Tan, Bill Thelin, M. Tyler Nelson, Jose Tarazona, John Wambaugh, Jun-young Yang, Changwoo Yu, Suzanne Fitzpatrick
The webinar series and workshop titled Trust Your Gut: Establishing Confidence in Gastrointestinal Models–An Overview of the State of the Science and Contexts of Use was co-organized by NICEATM, NIEHS, FDA, EPA, CPSC, DoD, the Johns Hopkins Center for
Greta Babakhanova, Carl Simon Jr., Eugenia Romantseva
Biofabricated tissue-engineered constructs have the potential to transform personalized medicine. However, characterizing these constructs post-fabrication and throughout preclinical use remains challenging. On December 1, 2022, the National Institute of
Carl Simon Jr., Anne L. Plant, Catherine Zander, Erich Bozenhardt, Christina Celluzzi, David Dobnik, Melanie Grant, John Hughes, Uma Lakshmipathy, Kok-Seong Lim, Laura Montgomery, Hesham Nawar, Thiana Nebel, Linda Peltier, James Sherley, Rouzbeh Taghizadeh, Eddie Tan, Sandrine Vessillier
One of the most challenging aspects of cell and gene therapy products (CGTPs) is defining mechanism of action (MOA), potency and efficacy of the product. This perspective examines these concepts and presents helpful ways to think about them through the
Anthony Asmar, Zackery Benson, Adele Peskin, Mylene Simon, Joe Chalfoun, Michael Halter, Anne L. Plant
To facilitate the characterization of unlabeled induced pluripotent stem cells (iPSCs) during culture and expansion, we developed an AI pipeline for nuclear segmentation and mitosis detection from phase contrast images of individual cells within iPSC
Carl Simon Jr., Smitha Rao, James Dolgin, Stephen Farias, Samerender Hanumantharao
Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations
Eun Jin Lee, Paul Salipante, Anthony Kotula, Dana Graves, Stella Alimperti, Yoontae Kim
Epithelial cell function is modulated by mechanical forces imparted by the extracellular environment. The transmission of forces onto the cytoskeleton by modalities such as mechanical stress and matrix stiffness is necessary to address by the development
Greta Babakhanova, Anant Agrawal, Deepika Arora, Allison Horenberg, Jagat Budhathoki, Joy Dunkers, Joe Chalfoun, Peter Bajcsy, Carl Simon Jr.
In the field of tissue engineering, 3D scaffolds and cells are often combined to yield constructs that are used as therapeutics to repair or restore tissue function in patients. Viable cells are required to achieve the intended mechanism of action for the
The presence and impact of variability in cells as the source material for genome engineering are important to consider for the design, execution and interpretation of outcomes of a genome-engineering process. Variability may be present at the genotype and
Reut Shainer, Vardit Kram, Tina Kilts, Andrew Doyle, Inbal Shainer, Carl Simon Jr., Jinyang Zeng-Brouwers, Liliana Schaefer, Marian Young
Endochondral bone development and regeneration relies on activation and proliferation of periosteum derived cells (PDCs). Biglycan (Bgn), an extracellular matrix component, is known to be expressed in bone and cartilage, however little is known about its
Genome editing is a rapidly evolving biotechnology with the potential to transform many sectors of industry such as agriculture, biomanufacturing, and medicine. This technology is enabled by an ever-growing portfolio of biomolecular reagents that span the
Mary Clare McCorry, Marcie Black, Chrysanthi Williams, Greta Babakhanova, Sumona Sarkar, Nathan Swami, Katherine A. Mirica, Sarah Boermeester, Abbie Underhill
The use of engineered cells, tissues, and organs has the opportunity to change the way injuries and diseases are treated. Commercialization of these groundbreaking technologies has been limited in part by the complex and costly nature of their manufacture
We establish here a set of cytocentric principles to improve use of cells for regenerative medicine (RM) applications. The five cytocentric principles are cells need 1) full-time protection from contamination, 2) physiologic simulation, 3) proper
Camille Lombard-Banek, Kerstin Pohl, Edward Kwee, John T. Elliott, John E. Schiel
Mass Spectrometry (MS)-based proteomic measurements are uniquely poised to impact the development of cell and gene therapies. With the adoption of rigorous instrumental performance controls (PQs), large-scale proteomics can move from a research to a
Yang Song, Jiaoyue Long, Jeremiah Woodcock, Joy Dunkers, Hung Chun Lin, Douglas M. Fox, Xiaoling Liao, Yonggang Lv, Li Yang, Martin Chiang
This study experimentally substantiates the micromechanical compatibility between cell and substrate is essential for cells to achieve energetically favorable mechanotransduction that directs phenotypic transitions. The argument for this compatibility is
Rebecca Schugar, Christy Gliniak, Lucas Osborn, William Massey, Naseer Sangwan, Anthony Horak, Rakhee Banerjee, Danny Orabi, Robert Helsley, Amanda Brown, Amy Burrows, Chelsea Finney, Kevin Fung, Frederick Allen, Daniel Ferguson, Anthony Gromovsky, Chase Neumann, Kendall Cook, Amy McMillan, Jennifer Buffa, James Anderson, Margarete Mehrabian, Maryam Goudarzi, Belinda Willard, Tytus Mak, Andrew Armstrong, Garth Swanson, Ali Keshavarzian, Jose Carlos Garcia-Garcia, Zeneng Wang, Aldons Lusis, Stanley Hazen, Mark Brown
Obesity has repeatedly been linked to reorganization of the gut microbiome, yet to this point obesity therapeutics have been targeted exclusively toward the human host. Here we show that gut microbe-targeted inhibition of the trimethylamine N-oxide (TMAO)
Greta Babakhanova, Stephen Zimmerman, Laura Pierce, Sumona Sarkar, Nicholas Schaub, Carl Simon Jr.
Cell viability is an essential measurement for cell therapy products. One of the most common tests is Trypan blue (TB) dye exclusion where blue-stained cells are counted via imaging. Typically, live and dead cells are counted based on pixel intensities of
Eugenia Romantseva, Drew S. Tack, Nina Alperovich, David Ross, Elizabeth Strychalski
Cell-free systems are increasingly used for in vitro protein production and offer a promising alternative to traditional, cell-based biomanufacturing[1-6]. At the state-of-the-art, cell-free systems can provide a high-throughput platform for on-demand
Stephen Zimmerman, Carl Simon Jr., Greta Babakhanova
The AbsorbanceQ app converts brightfield microscope images into absorbance images that can be analyzed and compared across different operators, microscopes and time. Absorbance-based measurements are comparable, which is useful when the aim is to
Fred Walls, Danielle France, Ward L. Johnson, John Miles, Nikki Rentz, William Cordell, Shelley Kon, Ian Babson
A new low cost, multi-channel desktop digital instrument was developed which measures bacteria response when exposed to multiple antibiotics vs a control channel with no antibiotic in about an hour by detecting PM noise induced by their mechanical motion
We present the differences in cell migration patterns of endothelial cells when they physically interact with cancer cells through a porous membrane barrier (1.2 µm pore size). Cells were dielectrophoretically trapped on opposite sides of an 11 µm thick
Elijah Petersen, Cordula Hirsch, John T. Elliott, Peter Wick, Harald Krug, Leonie Aengenheister, Ali Arif, Alessia Bogni, Agnieszka Kinsner-Ovaskainen, Sarah May, Tobias Walser, Matthias Roesslein
Elijah Petersen, Andrew Nguyen, Jeffrey Brown, John T. Elliott, Amy Clippinger, John Gordon, Nicole Kleinstreuer, Matthias Roesslein
The use of in vitro assays to inform decision-making requires robust and reproducible results across studies, laboratories, and time. Experiments using positive control materials are an integral component of an assay procedure to demonstrate the extent to