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The CELL-FREE Workshop sought to identify and prioritize actionable steps towards more reproducible and comparable cell-free systems for practical applications in bioengineering and biomanufacturing.
We present a generic fabrication scheme to produce polymer microparticles with engineerable, complex shapes. The polymer particles are made from a polyethylene-glycol based hydrogel using a polydimethylsiloxane (PDMS) based molding technique. A simple
M. L. De Leoz, David L. Duewer, Scientists Multiple, Stephen E. Stein
Glycosylation is a topic of intense current interest in the development of biologic drugs since it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of NISTmAb, a monoclonal antibody
Mary Kombolias, Jan Obrzut, Michael T. Postek, Dianne L. Poster, Yaw S. Obeng
The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive. This limits the amount of data that can be obtained from an individual sample and renders it difficult to produce statistically
Joshua Hunsberger, Martha Lundberg, Julie Allickson, Claudia Zylberberg, Sarah Beachy, Carl Simon Jr.
This perspective article is inspired by a workshop entitled "Cell and Tissue Manufacturing Innovation" that was held at the Tissue Engineering and Regenerative medicine Society (TERMIS). Five main concepts are discussed: 1) the US National Academies' Forum
Timothy P. Quinn, Jolene D. Splett, Joseph D. McColskey, James Dawson, David Smith, Adam Himes, Daniel Cooke
The Transvenous Cardiac Leads Working Group of the Cardiac Rhythm Management Devices Committee of The Association for the Advancement of Medical Instrumentation (AAMI CRMD WG1) is developing a fatigue performance standard for cardiac device leads. The
Shinichiro N. Muramoto, J Greg Gillen, Cynthia J. Zeissler, Edward Garboczi
The size, shape, and spatial distribution of active pharmaceutical ingredient (API) particles are important physical characteristics of drug delivery systems that can affect the performance, stability, appearance, and even bulk properties of the end
Nancy Lin, Maria S. Ibrahim, Ahmed Ibrahim, Abdulrahman Balhaddad, Michael D. Weir, Franklin Tay, Thomas Oates, Hockin H. Xu, Mary A. Melo
Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored for modulation of biofilm growth. Here, we investigated the effect of
Edward Garboczi, Jack F. Douglas, Beatriz Pazmino, Ahmed M. Hassan, Somen Baidya
Recent advances in synthetic biomaterial microenvironment design has enabled researchers to mimic the natural in vivo environment of the cell and, therefore, quantify the effect of the environment on the morphologies of stem cells. Recently, the National
Danielle Schultz, Robert G. Brinson, Fahriye N. Sari, Jeffrey Fagan, Christina Bergonzo, Nancy Lin, Joy Dunkers
The structure and dynamics of Ag complexes derived from single stranded DNA (ssDNA) is less understood than their double stranded (dsDNA) counterparts despite their great promise as fluorescent biological probes and sensors. In this work, we seek new
Danielle E. Gorka, Nancy Lin, John M. Pettibone, Justin Gorham
The antimicrobial properties of silver nanomaterials (AgNM) have been exploited in various consumer applications, including textiles such as wound dressings. Understanding how these materials chemically transform throughout their lifecycle is necessary to
Poly(ADP ribose) polymerase 1 (PARP1) is a multifunctional DNA repair protein of the base excision repair pathway and plays a major role in the repair of DNA strand breaks and in replication and transcriptional regulation among other functions. Mounting
Max J. Lerman, Shinichiro Muramoto, Navein Arumugasaamy, Michael Van Order, Josephine Lembong, Anushka Gerald, John G. Gillen, John P. Fisher
Traditional 2D culture systems made of polystyrene (PS) require the addition of surface chemistry beyond the nominal phenyl groups present to facilitate and encourage cell adhesion. Determining the surface properties which enhance protein adhesion from
Heidrun Wabnitz, Lin Yang, Rainer Macdonald, Jeeseong C. Hwang
In the clinically relevant field of tissue oximetry, there is an urgent need to develop phantom-based methods for validation. Physical phantom approaches based on solid or liquid turbid media containing hemoglobin with variable oxygenation have limited