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Becky Steffen

Ms. Becky Hill received her bachelor of arts at The University of Virginia in Biology in 1998 and then received her master of science at George Mason University in Molecular Biology in 2005. Upon graduation from UVA, she worked for the American Red Cross for two years doing research for the Plasma Derivatives division. She then went on to work for a small biotech company called Clearant for 5 years doing research with blood proteins. After receiving her masters at GMU, she began working for the Human Identity Project Team at NIST. She has been working in this group for 4 years mainly focusing on the following projects:

  1. Development and characterization of additional miniSTR loci for human identity testing
  2. Design and development of a large single amplification STR multiplex assay called the 26plex
  3. Low copy number DNA testing and analysis
  4. SRM 2391b update for additional STR loci, involving DNA sequencing and genotyping
  5. Beta-testing various human identity kits for forensic industry companies

Selected Publications

Butler, J.M., Coble, M.D., Decker, A.E., Duewer, D.L., Hill, C.R., Kline, M.C., Redman, J.W., Vallone, P.M. (2006) Setting standards and developing technology to aid the human identity testing community. Progress in Forensic Genetics 11, Elsevier Science: Amsterdam, The Netherlands, International Congress Series
1288, 628-635.

Coble, M.D., Hill, C.R., Vallone, P.M., Butler, J.M. (2006) Characterization and performance of new miniSTR loci for typing degraded samples. Progress in Forensic Genetics 11, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1288, 504-506.

Hill, C.R., Kline, M.C., Mulero, J.J., Lagace, R.E., Chang, C.-W., Hennessy, L.K., Butler, J.M. (2007) Concordance study between the AmpFlSTR MiniFiler PCR Amplification Kit and conventional STR typing kits. J. Forensic Sci. 52(4): 870-873.

Butler, J.M., Hill, C.R., Decker, A.E., Kline, M.C., Reid, T.M., Vallone, P.M. (2007) New autosomal and Y-chromosome STR loci: characterization and potential uses. Proceedings of the Eighteenth International Symposium on Human Identification. See

Hill, C.R., Kline, M.C., Coble, M.D., Butler, J.M. (2008) Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples. J. Forensic Sci. 53(1):73-80.

Butler, J.M., Hill, C.R., Decker, A.E., Kline, M.C., Vallone, P.M. (2008) New autosomal STR loci. Forensic Science International: Genetics Supplement Series (Progress in Forensic Genetics 12) 1(1): 95-96.

Vallone, P.M., Hill, C.R., Butler, J.M. (2008) Demonstration of rapid multiplex PCR amplification involving 16 genetic loci. FSI Genetics 3(1): 42-45.

Hill, C.R., Butler, J.M., Vallone, P.M. (2009) A new 26plex assay for use in human identity testing. J. Forensic Sci. (in press).


Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines

Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah L. Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin Hall, Mark Ladd, Megan Shaffer, Heath Berg, Jinliang Li, Georges Wigger, Steven P. Lund, Carolyn R. Steffen, Barbara Fransway, Bob Geraghty, Manuela Natoli, Beth Bauer, Susanne M. Gollin, Dale Lewis, Yvonne A. Reid
The Consortium for Mouse Cell Line Authentication was formed to validate Short Tandem Repeat (STR) markers for intraspecies identification of mouse cell lines

Development and Interlaboratory Evaluation of a NIST Reference Material RM 8366 for EGFR and MET Gene Copy Number Measurements

Hua-Jun He, Biswajit Das, Megan H. Cleveland, Chen Li, Corinne Camalier, Liang-Chu Liu, Kara L. Norman, Andrew Fellowes, Christopher McEvoy, Steven P. Lund, Jamie L. Almeida, Carolyn R. Steffen, Chris Karlovich, P. M. Williams, Kenneth D. Cole
The National Institute Standard and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR

Sequence-based U.S. population data for 27 autosomal STR loci

Katherine B. Gettings, Lisa A. Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter M. Vallone
This manuscript reports STR sequence-based allele frequencies for the "NIST 1036" sample set across 27 autosomal STR loci: D1S1656, TPOX, D2S441, D2S1338

Sequence-based U.S. population data for the SE33 locus

Lisa A. Borsuk, Katherine B. Gettings, Carolyn R. Steffen, Kevin M. Kiesler, Peter M. Vallone
A set of 1036 U.S. Population Samples were sequenced using the Illumina ForenSeq DNA Signature Prep Kit. This sample set has been highly characterized using a
Created October 9, 2019