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Projects/Programs

Displaying 1 - 9 of 9

Flow Cytometry Lab

Ongoing
Current Capabilities ERF value assignment measurement service for traceable ERF value assignment to microparticles, Aseptic sorting of cell lines, and primary cells Quantitative measurements of cell surface and intracellular biomarkers by estimating antibody bound per cell Accurate enumeration of

NIST Flow Cytometry Standards Consortium 

Ongoing
Advances in cell and gene-based therapeutics as well as other regenerative medicine products have increased the need for high quality, robust, and validated measurements for cell characterization. Flow cytometry, including imaging cytometry, has emerged as an important platform due to its ability to

Optofluidic Cytometry

Ongoing
Flow cytometers are commercial instruments that provide rapid multiparameter measurements of single cells, which makes them indispensable in basic research and clinical studies of diseases and drug therapies. Measurements of cellular attributes can include indicators of cell size and shape and well

Quantitative, Cell-based Immunofluorescence Assays

Completed
Analysis of signaling events within individual cells with microscopy imaging has several advantages over non-imaging techniques. The principal advantage of imaging cytometry over plate reader assays and even other single cell techniques such as flow cytometry is the potential for collection of

Quantitative Flow Cytometry Measurements

Ongoing
Flow cytometry is an essential tool for: basic biotechnological and immunological research, the clinical discovery of potential therapeutics, development and approval of drugs and devices, disease diagnosis, and therapeutic treatment and monitoring For example, flow cytometry is commonly used in pre

RMAT Laboratory Programs

Ongoing
Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not

Serology and Neutralization Assays for COVID-19

Ongoing
Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have
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