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Standards

Reference materials

Projects/Programs

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Development of a Collagen Fibril Reference Material for Reproducible Cell Response

Ongoing

Research is ongoing to assess what information is required to validate films of collagen as reference extracellular matrix. During the first portion of this work we will develop protocols for characterizing the prototype collagen reference material with various instrumental techniques to develop

Development of Methods for Measurement of Vitamin D and its Metabolites in Foods, Fortified Foods, and Dietary Supplements

Ongoing

Vitamin D exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D3 is produced in the body after sun exposure and is also the form used in most fortified foods. Dietary supplements may contain either vitamin D2 or D3. Measurement of vitamin D in foods and dietary

Figure contains text “Omics Data Quality” and human outline with arrows pointing from the human to the following specimen types: liver, serum, stool, plasma, urine. On either side of the human are analytical spectra and everything is surrounded by a decorative molecule structure circular border.

Development of NIST Reference Materials for Untargeted Metabolomics, Lipidomics and Proteomics

Ongoing

There is a significant need for tools to increase harmonization, comparability, and reproducibility between laboratories and/or studies, and to provide quality control of sample preparation and data analysis protocols utilized in untargeted omics analysis. Reference materials are being developed to

Human Vitamin D Binding Protein in complex with 25-hydroxyvitamin D3

Development of Reference Materials and Methods for Vitamin D Binding Protein

Ongoing

Vitamin D is essential for bone health, and vitamin D deficiency can lead to rickets in children and osteomalacia (soft bones) in adults. Vitamin D status is typically monitored through measurement of the metabolite 25-hydroxyvitamin D [25(OH)D] in serum. Most 25(OH)D is tightly bound to its

Development of Reference Measurement Procedures and Reference Materials for Creatinine

Ongoing

Kidney function can be evaluated by determining the glomerular filtration rate (GFR), which provides an estimate of how well the kidneys are removing waste and excess fluid from the blood. The GFR is not measured directly. Instead, a measurement of serum creatinine is performed, and this value is

Development of Reference Methods and Reference Materials for the Determination of Cardiac Troponin I in Human Serum

Ongoing

The clinical measurement of serum cardiac troponin I (cTnI) has become one of the most important tools in the diagnosis of acute myocardial infarction and myocardial damage. Unfortunately, considerable variability in clinical cTnI assay results is observed when comparing assays from different

NIST researcher pipetting solutions into 96-well plate for analysis

Development of Reference Methods and Reference Materials for Protein Biomarkers

Ongoing

Accuracy and reliability of clinical assays is essential for timely and accurate medical interventions. However, studies have shown that large variations in results can occur among routine clinical assays. In one example, a clinical comparison among 13 commercial cardiac troponin I assay platforms

Photograph of SRM 2669 Arsenic Species in Frozen Human Urine, with vials and desiccant packs, and labeled mylar bag.

Development of SRM 2669 for Accurate Assessment of Arsenic Exposure

Ongoing

Arsenic exposure in the U.S. population is an important part of the NHANES program conducted by CDC because dietary exposure to arsenic can cause serious health effects. A major challenge in the arsenic exposure assessment is that arsenic exists as various species in nature, and the toxicity of

Development of SRM 955c Toxic Elements in Caprine Blood

Completed

Intended Impact Over the last three decades, NIST, with support from the Centers for Disease Control (CDC), and in conjunction with the New York State Department of Health’s (NYSDOH) Wadsworth Center, has produced a number of frozen whole blood certified reference materials containing endogenous

Development of SRMs 2668 and 3668 for Accurate Assessment of Exposure to Toxic Elements and Anions

Completed

Toxic elements in the environment are a threat to public health and safety. Federal and state regulations have been issued to limit and abate the presence of toxic elements in the environment. The levels of toxic elements in the U.S. population are carefully monitored. NHANES is one of the federal

Development of Standards for the World Meteorological Organization, Global Atmosphere Watch Program

Ongoing

Non-methane hydrocarbons (NMHCs) are emitted into the atmosphere by many biogenic and anthropogenic sources that include automobiles and other transportation vehicles, biomass burning, and oceans. In urban environments in particular NMHCs are important because they are precursors and contributors to

Dietary Supplement Laboratory Quality Assurance Program (DSQAP)

Ongoing

Approximately 75% of the U.S. population takes dietary supplements, including vitamins and mineral supplements, representing an annual expenditure of more than $20 billion. Regulations, driven by reported cases of inaccurate labeling, adulteration, contamination (with pesticides, heavy metals, or

Dietary Supplement Laboratory Quality Assurance Program (DSQAP) Consortium

Ongoing

The objective of the DSQAP Consortium is to develop and evaluate measurement methods and standards to support quality and safety for the dietary supplement testing community. Approximately 75% of the U.S. population takes dietary supplements, including vitamins and mineral supplements, representing

Picture of PBD set up for wavelength measurement

Diffraction Metrology and Standards

Ongoing

Diffraction techniques can provide data on a number of sample characteristics. Therefore, the method of certification and the artifact itself are chosen to address a specific measurement issue pertinent to a diffraction experiment. NIST diffraction SRMs may be divided into five groups: Line Position

Digital PCR

Ongoing

With appropriately validated assays, digital polymerase chain reaction (dPCR) determines the number of DNA targets (copies) per reaction partition, without the need for a standard curve. Reaction partitions can be in the form of fixed chambers in a microfluidic device, referred to as chamber digital

DNA Mixture Interpretation

Ongoing

Interlaboratory Studies Mixture interlaboratory studies provide a means to assess a laboratory's ability to process and interpret DNA mixtures. Initial ‘Mixed Stain’ studies (published in 2001) provided physical samples for DNA profiling with the first commercial STR typing tests. Later, mixture

Establishing Measurement Traceability for Gaseous Mercury Emissions Monitoring

Ongoing

On May 18, 2005 the EPA promulgated the Clean Air Mercury Rule (CAMR), an amendment to the Clean Air Act, which established performance standards for the mercury output of new and existing coal-fired power plants. The rule was designed to significantly reduce mercury emissions from coal-fired power

Evaluation of Independent High-Precision Assay Procedures for a High-Purity Primary Standard Reagent

Ongoing

The NIST high-purity primary standard reagent SRMs are the benchmark for a wide variety of acidimetric and oxidation-reduction titrations. This suite of SRMs includes SRM 84k Potassium Hydrogen Phthalate , SRM 350b Benzoic Acid , and SRM 973 Boric Acid , each used as a standard acid. SRM 723d Tris

FAQs - Genome in a Bottle

Ongoing

Photograph of SRM 2378 Fatty Acids in Human Serum showing three vials labeled Serum 1, Serum 2, and Serum 3.

Fatty Acids in Human Serum and Plasma Quality Assurance Program (FAQAP)

Completed

Interlaboratory comparison studies of the FAQAP focused on the measurement of 24 fatty acids in selected serum and plasma matrices. Frozen or freeze-dried serum or plasma samples were sent to laboratories for analysis together with control materials. Results were returned to NIST for data evaluation

Fluid Suspensions and Emulsions

Ongoing

We aim to: Develop measurement capabilities to relate solution structure and viscosity through novel combinations of unique small volume, high shear rate rheometers with scattering and particle-imaging techniques. A major application of these capabilities is understanding self-association in fluids

Food Nutrition and Safety Measurements Quality Assurance Program (FNSQAP)

Ongoing

As part of a revamp of QAPs in 2021, NIST established the FNSQAP to support the NIST Food Safety Program and Measurements and Standards to Support Nutrition Labeling. Participants measure nutrient components (e.g., minerals, vitamins, fatty acids, dietary fiber) and contaminants (e.g., toxic

The inside of a DNA extraction robot hold tube in a rack

Forensic Genetics

Ongoing

Since the late 1980s, NIST has had scientists involved in DNA testing (aka "DNA Fingerprinting"). Early concerns over measurement accuracy and poor quality control of forensic DNA tests caused the Department of Justice to call upon NIST scientists to help with standards development and technology

Fossil Fuel Standard Reference Materials

Ongoing

The regulatory requirements with respect to air quality and fossil fuel specifications are continually changing, and consequently the associated measurement needs of the fossil fuel energy industries also change. For example, in recent years there has been a dramatic reduction in the allowable

Genome in a Bottle

Ongoing

Consortium goals: The Genome in a Bottle Consortium is a public-private-academic consortium hosted by NIST to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice and innovations

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