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Cell biology


Traditionally, cell biology has been an academic subject area focused on the basic science of mammalian cells and single cell organisms like bacteria. Today, the quantitative application of cell biology is a segue to advanced manufacturing. 

In the past couple of decades, the field of cell biology has incorporated many technological advances.  These include whole genome sequencing, single cell RNA transcriptomics, sub-diffraction resolution of intracellular fluorescent probes, precision gene editing, and others. The “omics” methods and advances in large-scale cell imaging have enabled the accumulation of big datasets suitable for advanced mathematical analysis.

With these new tools, we are entering an era of cell biology where it will be possible to understand and predict the complex regulatory mechanisms that determine cell function, the “missing link” between basic science and useful therapeutics[i] and other bioeconomy products.  With that understanding of the relationships between structure and function will come an increased ability to design, control, and manufacture cells.

 At NIST, we are developing systems that will lead to better quantification and prediction of complex biological processes for improved drug development, personalized medicine, cell therapies, and the design and manufacturing of cells and cell products for the health, agriculture, and environment sectors.

Selected Programs and Accomplishments

Quantifying dynamic biological processes with live cell imaging.  Cells can respond to a combination of many extra-cellular signals, including chemical, mechanical, electrical and optical, and process these signals through multistep biochemical reactions. The stochastic nature of signal processing in cells results in a range of responses. Accurate characterization of a heterogeneous population of cells requires sampling a statistically significant number of individual cells.  Ultimately, understanding the response functions of cells will allow us to predict the efficacy of a product or the outcome of a manufacturing process change. At NIST, high speed image data acquisition from living cells is one technology being used to collect the quantitative dynamic data needed to develop predictive models. 

Thermodynamic principles describe and predict cell populations.  Biological processes can be described by physical relationships, although much work is still needed in this area. We model the heterogeneity of cells as a population of microstates and apply thermodynamic principles for predicting population response.

Non-invasive stem cell imaging made possible with deep learning and very large image datasets.  Imaging of living cells is a powerful tool for characterization and prediction since it provides quantitative data on morphological and chemical features of cells, as well as temporal and spatial information.  Fluorescent labels and gene reporters provide a window into specific protein machinery of the cell but require radiation that can be damaging. We use fluorescent probes in cells to train neural networks to segment and track stem cells in culture that are imaged under nonfluorescent (bright field) illumination. This approach, in principle, will enable quality control of live, unlabeled cells with minimum perturbation during manufacturing. 

Improving Metrics for Cell-Based Therapies and Regenerative Medicines.  This is one of the most exciting and challenging applications of advanced cell biology. Some cell-based therapies have been shown to cure, not just to treat, previously deadly cancers. At NIST, we are developing technologies that will enable better characterization of cell therapy products and processes. One approach uses the creation of reporter cell lines which are engineered to produce fluorescent reporter molecules when important control pathways are induced in cells.  This approach will allow real time monitoring of a manufacturing protocol.


[i] Berger and Iyengar, 2009


A woman in blue coveralls and purple gloves works at a lab table.
Credit: R. Wilson/NIST

Meet Greta Babakhanova, a postdoctoral researcher here at NIST with boundless drive and a passion for reducing human suffering. It’s an ambitious goal that, for her, spans multiple chapters of her professional life and means entering the fast-growing fields of tissue engineering and regenerative medicine.

Chapter I: Human cells don’t grow randomly (aka Greta’s academic adventure).

Scientists need to grow cells with structures like the frames (or scaffolds) that you might see around buildings under construction. In her Ph.D. at Kent State University, Greta worked with liquid crystals (a state of matter between a liquid and a solid) to create a coating for those scaffolds that she could dynamically control with temperature and light. With those stimuli, the molecules in the liquid crystal coating aligned in whatever direction Greta wanted, creating surface patterns that particles or biological cells could adhere to and grow on.

Chapter II: Human cells don’t grow alone (aka Greta’s NIST experience).

In common practices for counting cells, scientists examine samples of cells that float freely and unattached in a liquid, unlike in reality, where they come together into a 3D structure and shape. Researchers also often use toxic dyes for labeling live and dead cells. These dyes kill the cells and essentially end the experiment. Greta joined a team at NIST in the midst of developing a new technique to count and identify living cells in 3D scaffolds. With help from optical imaging equipment from the U.S. Food and Drug Administration, she and her team are up for the challenge of finding ways to bypass those obstacles.

Chapter III: TBD (aka the future awaits).

Scientists can combine cells and the scaffolds they grow on into structures that can repair or restore damaged or lost tissue or organs in patients. That’s where translational research — taking advances out of the lab and into clinical studies — comes into play, and that’s where Greta is headed next. One day, she hopes to apply her experience with 3D scaffolds at NIST to the clinical field, using a toolbox of biomaterials like the liquid crystals from her Ph.D. days.

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News and Updates

Projects and Programs

Cancer Genome in a Bottle

Part of the Genome in a Bottle Consortium hosted by NIST dedicated to authoritative characterization of benchmark cancer genomes. Complements NIST's current DNA

Tissue Engineering Measurands

Cell viability is often measured as a quality metric of tissue engineered medical products. However, there are a number of inherent assumptions about cell


Mechanical Regulation of Oral Epithelial Barrier Function

Eun Jin Lee, Yoontae Kim, Paul Salipante, Anthony Kotula, Dana Graves, Stella Alimperti
Epithelial cell function is modulated by mechanical forces imparted by the extracellular environment. The transmission of forces onto the cytoskeleton by