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Search Publications by: Monique Hunter (Fed)

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Displaying 1 - 8 of 8

A mathematical framework to correct for compositionality in microbiome datasets

January 6, 2026
Author(s)
Samuel Forry, Stephanie Servetas, Jason Kralj, Monique Hunter, Jennifer Dootz
The increasing use of metagenomic sequencing (MGS) for microbiome analysis has significantly advanced our understanding of microbial communities and their roles in various biological processes, including human health, environmental cycling, and disease

Measurement quality metrics to improve absolute microbial cell counting

August 18, 2025
Author(s)
Kirsten Parratt, David Newton, Joy Dunkers, Jennifer Dootz, Monique Hunter, Alshae Logan, Laura Pierce, Sumona Sarkar, Stephanie Servetas, Nancy Lin
Total and viable microbial cell counts are increasingly important for applications including live biotherapeutic products, food safety, and probiotics. In microbiology, cells are quantified using methods such as colony forming unit (CFU), flow cytometry

A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements

January 14, 2025
Author(s)
Samuel Forry, Stephanie Servetas, Jennifer Dootz, Monique Hunter, Jason Kralj, James Filliben, Scott Jackson
The particular experimental methods employed during metagenomic sequencing analyses of microbiome samples can significantly impact the resulting data but typically vary substantially between laboratories. In this study, a full factorial experimental design

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Author(s)
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Performance of NIST SRM(R) 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

February 10, 2022
Author(s)
Jessica Willis, Mano Sivaganesan, Richard Haugland, Jason Kralj, Stephanie Servetas, Monique Hunter, Scott Jackson, Orin Shanks
Fecal pollution remains a significant challenge for recreational water quality management worldwide. In response, there is a growing interest in the use of real-time quantitative PCR (qPCR) methods to achieve same-day notification of recreational water
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