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Search Publications by: David P. Hoogerheide (Fed)

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Displaying 1 - 25 of 28

Restricting a-Synuclein Transport into Mitochondria by Inhibition of a-Synuclein-VDAC Complexation as a Potential Therapeutic Target for Parkinson's Disease Treatment

October 1, 2022
Megha Rajendran, Maria Queralt-Martin, Philip A. Gurnev, William M. Rosencrans, Amandine Rovini, Daniel Jacobs, Kaitlin Abrantes, David Hoogerheide, Sergey M. Bezrukov, Tatiana Rostovtseva
Involvement of alpha-synuclein (αSyn) in Parkinson's disease (PD) is complicated and difficult to trace on cellular and molecular levels. Recently we established that αSyn can regulate mitochondrial function by voltage-activated complexation with the

The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating

August 17, 2022
Van Ngo, Maria Queralt-Martin, Farha Khan, Lucie Bergdoll, Jeff Abramson, Sergey M. Bezrukov, Tatiana Rostovtseva, David Hoogerheide, Sergei Noskov
The voltage-dependent anion channel (VDAC) is a β-barrel channel of the mitochondrial outer membrane (MOM) that passively transports ions, metabolites, polypeptides, and single-stranded DNA. VDAC responds to a transmembrane potential by transitioning to a

Low-Background Neutron Reflectometry from Solid/Liquid Interfaces

February 1, 2022
David Hoogerheide, Joseph Dura, Brian B. Maranville, Charles F. Majkrzak
Liquid cells are an increasingly common sample environment for neutron reflectometry experiments and are critical for measuring the properties of materials at solid/liquid interfaces. Background scattering determines the maximum useful scattering vector

Highly Basic Clusters in the Herpes Simplex Virus 1 Nuclear Egress Complex Drive Membrane Budding by Inducing Lipid Ordering

August 24, 2021
Michael K. Thorsen, Alex Lai, Michelle W. Lee, David Hoogerheide, Gerard C. Wong, Jack H. Freed, Ekaterina Heldwein
During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This process is mediated by the viral nuclear egress complex (NEC), but how the NEC interacts with lipids or generates

VDAC Gating Thermodynamics, but Not Gating Kinetics, Are Virtually Temperature Independent

December 15, 2020
Maria Queralt-Martin, David Hoogerheide, Sergei Y. Noskov, Alexander M. Berezhkovskii, Tatiana Rostovtseva, Sergey Bezrukov
The voltage-dependent anion channel, VDAC, is the most abundant protein in the mitochondrial outer membrane and an archetypical β-barrel channel. Here, we study the effects of temperature on VDAC channels reconstituted in planar lipid membranes at the

Advances in Translational Nanotechnology: Challenges and Opportunities

May 28, 2020
Shyam S. Mohapatra, Robert D. Frisina, Subhra Mohapatra, Kevin B. Sneed, Eleni Markoutsa, Tao Wang, Rinku Dutta, Ratka Damnjanovic, Manh-Huong Phan, Daniel J. Denmark, Manas R. Biswal, Andrew R. McGill, Ryan Green, Mark Howell, Payal Ghosh, Alejandro Gonzalez, Nadia Tasnim Ahmed, Brittney Borresen, Mitchell Farmer, Melissa Gaeta, Krishna Sharma, Christen Bouchard, Danielle Gamboni, Jaime Martin, Bianca Tolve, Mandip Singh, Jack W. Judy, Chenzhong Li, Swadeshmukul Santra, Sylvia Daunert, Elnaz Zeynaloo, Ryan M. Gelfand, Steven Lenhert, Eric S. McLamore, Dong Xiang, Victoria Morgan, Lisa E. Friedersdorf, Ratnesh Lal, Thomas J. Webster, David Hoogerheide, Thanh Duc Nguyen, Martin J. D'Souza, Mustafa Culha, Pierre P. Kondiah, Donald K. Martin

Protein Translocation Activity in Surface-Supported Lipid Bilayers

August 25, 2019
Kanokporn Chattrakun, David Hoogerheide, Chunfeng Mao, Linda L. Randall, Gavin M. King
Surface-supported lipid bilayers are used widely throughout the nanoscience community as robust mimics of cellular membrane. For example, they are frequently employed in single molecule atomic force microscopy (AFM) studies to shed light on membrane

Structural Investigations of Membrane-Associated Proteins by Neutron Reflectometry

July 1, 2019
Rebecca Eells, David Hoogerheide, Paul A. Kienzle, Mathias Loesche, Charles Majkrzak, Frank Heinrich
Neutron reflectometry is a powerful technique for probing the structure of lipid bilayer membranes and membrane-associated proteins. Measurements of the specular neutron reflectivity as a function of momentum transfer can be performed in aqueous

Probing Membrane Association of a-Synuclein Domains with VDAC Nanopore Reveals Unexpected Binding Pattern

March 14, 2019
Daniel Jacobs, David Hoogerheide, Amandine Rovini, Zhiping Jiang, Jennifer C. Lee, Tatiana K. Rostovtseva, Sergey M. Bezrukov
It is well established that α-synuclein (α-syn) binding from solution to the surface of membranes composed of negatively charged and/or non-lamellar lipids can be characterized by equilibrium dissociation constants of tens of micromolar. Previously, we

Porous Zero-Mode Waveguides for Picogram-Level DNA Capture

February 13, 2019
Vivek Jadhav, David Hoogerheide, Jonas Korlach, Meni Wanunu
We have recently shown that nanopore zero-mode waveguides are effective tools for capturing picogram levels of long DNA fragments for single-molecule DNA sequencing. Despite these key advantages, manufacturing of large arrays is not practical due to the

Optimization of Reflectometry Experiments using Information Theory

February 1, 2019
Bradley W. Treece, Paul A. Kienzle, David Hoogerheide, Charles Majkrzak, Mathias Loesche, Frank Heinrich
A method based on Bayesian statistics and information theory is developed to analyze the information gain Δ}H of surface-sensitive reflectometry experiments. After presenting the underlying mathematical framework and its implementation, the method is

Real-Time Nanopore-Based Recognition of Protein Translocation Success

February 27, 2018
David Paul Hoogerheide, Philip A. Gurnev, Tatiana K. Rostovtseva, Sergey M. Bezrukov
A growing number of new technologies are supported by a single- or multi-nanopore architecture for capture, sensing, and delivery of polymeric biomolecules. 1-6 Nanopore-based single-molecule DNA sequencing, in which engineered nanopores 7 are augmented

Structural Features and Lipid Binding Domain of Tubulin on Biomimetic Mitochondrial Membranes

April 18, 2017
David Paul Hoogerheide, Sergei Y. Noskov, Daniel Jacobs, Lucie Bergdoll, Vitalii Ivanovich Silin, David L. Worcester, Jeff Abramson, Hirsh Nanda, Tatiana K. Rostovtseva, Sergey M. Bezrukov
Amphitropic proteins, the subfamily of peripheral membrane proteins that interact directly with lipid bilayers, comprises members whose function depends on their localization either in the cytosol or at the cellular membranes. Despite the well-established