Porous Zero-Mode Waveguides for Picogram-Level DNA Capture

Published

February 13, 2019

Author(s)

Vivek Jadhav, David Hoogerheide, Jonas Korlach, Meni Wanunu

Abstract

We have recently shown that nanopore zero-mode waveguides are effective tools for capturing picogram levels of long DNA fragments for single-molecule DNA sequencing. Despite these key advantages, manufacturing of large arrays is not practical due to the need for serial nanopore fabrication. To overcome this challenge, we have developed an approach for water-scale fabrication of waveguide arrays on low-cost porous membranes, which are deposited using molecular layer deposition. The network of serpentine pores that comprise the membrane at each waveguide base allows efficient DNA capture at picogram levels while eliminating the need for prohibitive serial pore milling. Here, we show that loading efficiency of these porous waveguides is up to two orders of magnitude greater than their nanopore predecessors. This new device facilitates scaling up the process, greatly reducing the cost and effort of manufacturing. Further, the porous zero-mode waveguides can be used for applications which benefit from low-input single molecule real-time (SMRT) sequencing.

Citation

Nano Letters

Volume

19

Issue

2

Pub Type

Journals

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Keywords

nanopores and nanoporous materials, zero-mode waveguides, DNA manipulation and capture, neutron reflectometry, molecular layer deposition, DNA sequencing

Citation

Jadhav, V. , Hoogerheide, D. , Korlach, J. and Wanunu, M. (2019), Porous Zero-Mode Waveguides for Picogram-Level DNA Capture, Nano Letters, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=926859 (Accessed October 13, 2025)