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Search Publications by: Illarion Turko (Fed)

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Displaying 26 - 43 of 43

Expression and characterization of 15N-labeled human C-reactive protein in Escherichia coli and Pichia pastoris for use in isotope-dilution mass spectrometry

September 1, 2012
Author(s)
Eric L. Kilpatrick, Wei-Li Liao, Johanna Camara, Illarion V. Turko, David M. Bunk
Isotope dilution mass spectrometry using stable isotope labeled internal standards is unsurpassed in its ability to quantify analytes with accuracy and low measurement uncertainty. The extension of this technique to proteins relies upon the expression of

Mass spectrometry quantification of clusterin in the human brain

August 20, 2012
Author(s)
Junjun J. Chen, Meiyao M. Wang, Illarion V. Turko
A multifunctional glycoprotein clusterin has been associated with late-onset Alzheimer’s disease (AD). This finding should prompt further investigation to define the role of clusterin in AD phenotypes. For that reason, the development of techniques to

Protein characterization of a candidate mechanism SNP for Crohn's disease: the macrophage stimulating protein R689C substitution

November 7, 2011
Author(s)
Illarion V. Turko, Natalia Gorlatova, Kinlin L. Chao, Lipika Ray, Rawan Araj, Andrey Galkin, John Moult, Osnat Herzberg
Macrophage Stimulating Protein (MSP) is the ligand of RON receptor tyrosine kinase. MSP binding to RON activates a signaling pathway that plays a dual role in inflammation, initially stimulating macrophage chemotaxis and phagocytosis, but also changing

Quantification of cholesterol-metabolizing P450s CYP27A1 and CYP46A1 in neural tissues reveals a lack of enzyme-product correlations in human retina but not human brain

January 7, 2011
Author(s)
Wei-Li Liao, Gun-Young Heo, Nathan G. Dodder, Rachel Reem, Natalia Mast, Suber Huang, Pier L. DiPatre, Illarion Turko, Irina A. Pikuleva
Improper cholesterol metabolism in the brain causes neurological diseases. Factors affecting cholesterol homeostasis includes cholesterol hydroxylation reactions, which are regulated by cytochrome P450 enzymes. One approach to estimate the total enzymatic

Optimizing the Conditions of a Multiple Reaction Monitoring Assay for Membrane Proteins: Quantification of Cytochrome P450 11A1 and Adrenodoxin Reductase in Bovine Adrenal Cortex and Retina

July 1, 2010
Author(s)
Illarion V. Turko, Wei-Li Liao, Gun-Young Heo, Nathan G. Dodder, Irina A. Pikuleva
Although 30% of naturally occurring proteins are predicted to be embedded in biological membranes, membrane proteins are traditionally understudied due to limitations of the available analytical tools. To be applicable to the analysis of membrane proteins

Accumulation of Large Protein Fragments in Prematurely Senescent ARPE-19 Cells

October 1, 2009
Author(s)
Illarion V. Turko, Wei-Li Liao
Senescence of retinal pigment epithelial (RPE) cells is a crucial event in the pathogenesis of age-related macular degeneration (AMD). This study was design to improve the understanding of changes that underlie the RPE senescence. The levels of various

A new approach to assess mAb aggregation

January 1, 2001
Author(s)
Illarion Turko
A proof-of-concept for new methodology to detect and potentially quantify mAb aggregation is presented. Assay development included using an aggregated mAb as bait for screening of a phage display peptide library and identifying those peptides with random

Quantitative Proteomic Profiling of Prematurely Senescent ARPE-19 Cells

January 1, 2001
Author(s)
Illarion Turko, Wei-Li Liao
Senescence of retinal pigment epithelial (RPE) cells is a crucial event in the pathogenesis of age-related macular degeneration (AMD). ARPE-19 is a nontransformed human RPE cell line that displays many differentiated properties typical of RPE in vivo. The