Publications

Displaying 76 - 82 of 82

Driven DNA Transport Into an Asymmetric Nanometer-Scale Pore

October 2, 2000

Author(s)

S. E. Henrickson, Martin Misakian, B Robertson, John J. Kasianowicz

To understand the mechanism by which individual DNA molecules partition into a nanometer-scale pore, we studied the concentration and voltage dependence of polynucleotide-induced current blockades of single a-hemolysin ionic channels. At fixed single

Diffusion Bias and Photophysical Dynamics of Single Molecules in Unsupported Lipid Bilayer Membranes Probed with Confocal Microscopy

July 1, 2000

Author(s)

D L. Burden, John J. Kasianowicz

We utilize scanning confocal fluorescence microscopy to interrogate single fluorescent lipid molecules in unsupported planar bilayers. At high photon flux, an optical trapping effect appears that is approximately 100,000 times larger than predicted by

Interrogating Unsupported Lipid Bilayers With Scanning Confocal Single-Molecule Fluorescence Microscopy

January 23, 2000

Author(s)

D L. Burden, John J. Kasianowicz

We describe the use of scanning confocal fluorescence microscopy to probe the structure and temporal dynamics of unsupported planar lipid bilayer membranes. At high fluorescent label concentration, the shape and stability of the membranes are evaluated

Electrostatically Driven Polymer Translocation Through a Narrow Pore

January 1, 2000

Author(s)

Z Konkoli, T Ambjornsson, S P. Apell, John J. Kasianowicz

We studied theoretically how single-stranded DNA enters a nanometer-scale pore. We describe the flux of DNA molecules into the pore as a function of the applied electrostatic potential. The number of polynucleotide segments that entered the pore's entrance

Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules

December 1, 1999

Author(s)

M Akeson, D Branton, John J. Kasianowicz, E Brandin, D W. Deamer

Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential single

Dynamic Partitioning of Neutral Polymers into a Single Ion Channel

June 1, 1999

Author(s)

S. M. Bezrukov, John J. Kasianowicz

Polymers partitioning into highly confined spaces can be studied using single nanometer-scale pores formed by protein ion channels. The ionic conductance of a channel depends on the state of occupancy of the pore by polymer and serves as a measure of

Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane B-Barrel

February 1, 1999

Author(s)

John J. Kasianowicz, D L. Burden, L C. Han, S Cheley, H Bayley

We are exploring the ability of genetically engineered versions of the Staphlococcus aureus αHL} ion channel to serve as rationally designed sensor components for analytes including divalent cations. We show here that neither the hemolytic activity nor the

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