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Search Publications by Donald H. Atha

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Displaying 1 - 25 of 36

Controlled Potential Electrooxidation of Genomic DNA

January 11, 2018
Vytautas Reipa, Donald H. Atha, Sanem Hosbas Coskun, Christopher M. Sims, Bryant C. Nelson
Exposure of mammalian cells to oxidative stress can result in DNA damage that adversely affects many cell processes. We used bulk electrolysis in an electrochemical system and gas chromatographic mass spectrometric analysis (GC/MS/MS) to control and

The Comet Assay: Methods for Quantitative Image Analysis and Reproducibility

September 1, 2016
Donald H. Atha, Signe Braafladt, Vytautas Reipa
The comet assay,or single cell gel electrophoresis,is a sensitive and efficient technique for analyzing DNA damage in the form of single and double strand breaks in single cells. DNA alteration,from strand breaks,can lead to cancer and other health risks

Functionalization-Dependent Induction of Cellular Survival Pathways by CdSe Quantum Dots in Primary Normal Human Bronchial Epithelial Cells

September 5, 2013
Amber Nagy, Jennifer A. Hollingsworth, Bin Hu, Andrea Steinbruck, Peter C. Stark, Cristina Rios Valdez, Momchilo Vuyisich, Michael H. Stewart, Donald H. Atha, Bryant C. Nelson, Rashi Iyer
Quantum dots (QDs) are semiconductor nanocrystals exhibiting unique optical properties which can be exploited for many practical applications ranging from photovoltaics to biomedical imaging and drug delivery. They are increasingly manufactured in large

NIST Gold Nanoparticle Reference Materials Do Not Induce Oxidative DNA Damage

February 1, 2013
Bryant C. Nelson, Donald H. Atha, John T. Elliott, Bryce J. Marquis, Elijah J. Petersen, Danielle Cleveland, Stephanie S. Watson, I-Hsiang Tseng, Andrew Dillon, Melissa Theodore, Joany Jackman
Well-characterized, nanoparticle reference materials are urgently needed for nanomaterial toxicity studies. The National Institute of Standards and Technology has developed three gold nanoparticle (AuNP) reference materials (10 nm, 30 nm, 60 nm) to address

Copper oxide nanoparticle mediated DNA damage in terrestrial plant models

December 22, 2011
Bryant C. Nelson, Donald H. Atha, Elijah J. Petersen, Huanhua Wang, Danielle Cleveland, Richard D. Holbrook, Pawel Jaruga, M Miral Dizdar, Baoshan Xing
Engineered nanoparticles, due to their unique electrical, mechanical and catalytic properties, are presently found in many commercial products and will be intentionally or inadvertently released at increasing concentrations into the natural environment

Standards for Validation of Cancer Biomarkers

April 2, 2005
Catherine D. O'Connell, Donald H. Atha, J P. Jakupciak
Over the past 10 years, DNA testing to identify disease associated mutations and specific organisms, which represent public health and safety concerns, have become major targets for laboratory testing. RNA testing is also emerging for gene expression, both

Analytical Validation of Telomerase Activity for Cancer Early Detection: TRAP/PCR-CE and hTERT mRNA Quantification Assay for High-Throughput Screening of Tumor Cells

August 1, 2004
J P. Jakupciak, Wenhua Wang, Peter E. Barker, S Srivastava, Donald H. Atha
Activation of telomerase plays a critical role in unlimited proliferation and immortalization of cells. Telomerase activity has been shown to correlate with tumor progression, indicating that tumors expressing this enzyme posses aggressive clinical

Molecular Biomarkers Used to Detect Cellular/Genetic Damage in Tissue-Engineered Skin

January 1, 2004
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, H Rodriguez
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, Tissue engineered skin (TestSkin II) was obtained, separated into its two cellular layers

Biomarkers Used to Detect Genetic Damage in Tissue-Engineered Skin

January 1, 2003
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, M. Dizdaroglu, H Rodriguez
The goal of the research was to help identify cellular biomarkers and assess measurement technologies that will help manufacturers determine how cells react to inflammatory stimuli from different polymer matrixes and assure that tissue-engineered medical

High-Throughput Analysis of Telomerase by Capillary Electrophoresis

January 1, 2003
Donald H. Atha, K Miller, A D. Sanow, J Xu, J L. Hess, O C. Wu, Wenhua Wang, S Srivastava, W E. Highsmith
The enzyme telomerase is expressed in 85-90% of all human cancers, but not in normal, non-stem cell somatic tissues. Clinical assays for telomerase in easily obtained body fluids would have great utility as non-invasive, cost-effective methods for the

Biomarkers of TP53 Mutation and Y-Chromosome Loss Used to Detect Cellular/Genetic Damage in Tissue Engineered Skin

September 1, 2002
H Rodriguez, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell
Tissue engineering is an emerging area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. Fundamentals to many tissue-engineered

Standardization of PCR Amplification for Fragile X Trinucleotide Repeat Measurements

January 1, 2002
C D. O'Connell, Donald H. Atha, J P. Jakupciak, Kristy L. Richie
To provide the clinical diagnostics community with accurate protocols and measurements for the detection of genetic disorders, we have established a quantitative a quantitative measurement program for trinucleotide repeats associated with human disease. In

SSCP Analysis of Point Mutations by Multi-Color Capillary Electrophoresis

November 1, 1999
K Hayashi, H M. Wenz, M Inazuka, T Tahira, Donald H. Atha
Virtually all methods for the detection of mutations (polymorphism or variant) rely on polymerase chain reaction (PCR). Direct sequence determination of a PCR product is the gold standard for identifying mutaions. However, the vast majority of the signal

Analysis of Environmentally Induced DNA Damage by Capillary Electrophoresis

September 1, 1999
Z E. Nackerdien, B Siles, S Nivens, Donald H. Atha
Chemical pollutants, ionizing radiation, the products of aerobic metabolism and UV light are important metabolic and environmental factors contributing to DNA damage. Ionizing radiation, in paticular, induces damage in the form of single strand breaks