Biomarkers of TP53 Mutation and Y-Chromosome Loss Used to Detect Cellular/Genetic Damage in Tissue Engineered Skin
H Rodriguez, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell
Tissue engineering is an emerging area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. Fundamentals to many tissue-engineered devices are issues of inflammation associated with how biological cells respond to a given matrix or when inserted into the body. Because studies have shown that inflammatory-based oxidative DNA damage is mutagenic if not repaired by a cell's DNA repair mechanisms, the goal was to develop biomarkers that help assure that tissue-engineered products are free of permanent genetic changes that could occur during the manufacturing phase, storage or shipment of the product. The use of high performance liquid chromatography (DHPLC) and single strand conformation polymorphism capillary electrophoresis (CE-SSCP) technology was used to quantitatively measure the level of DNA point mutations along the TP53 gene (the most commonly mutaged gene in skin cancer). It was shown that the tissue engineered skin did not contain mutations in this gene at the level of sensitivity of DHPLC and CE-SSCP. A second set of biomarkers investigated was the loss of the Y-chromosome that is associated with long-term cell passage. Using Fluorescent In Situ Hybridization (FISH), no elevated levels of Y-chromosome loss was found in the tissue-engineered skin cells as compared to proper controls.
Southern Biomedical Engineering Conference | 21st | | Medical and Engineering Publshers
September 1, 2002
Southern Biomedical Engineering Conference
cellular biomarkers, tissue engineering
, Marino, M.
, McAndrew, P.
, Atha, D.
, Jaruga, P.
, Birincioglu, M.
, Barker, P.
and O'Connell, C.
Biomarkers of TP53 Mutation and Y-Chromosome Loss Used to Detect Cellular/Genetic Damage in Tissue Engineered Skin, Southern Biomedical Engineering Conference | 21st | | Medical and Engineering Publshers
(Accessed December 6, 2023)