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Search Publications by: Donald H. Atha (Assoc)

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Displaying 26 - 50 of 72

Standards for Validation of Cancer Biomarkers

April 2, 2005
Author(s)
Catherine D. O'Connell, Donald H. Atha, J P. Jakupciak
Over the past 10 years, DNA testing to identify disease associated mutations and specific organisms, which represent public health and safety concerns, have become major targets for laboratory testing. RNA testing is also emerging for gene expression, both

Analytical Validation of Telomerase Activity for Cancer Early Detection: TRAP/PCR-CE and hTERT mRNA Quantification Assay for High-Throughput Screening of Tumor Cells

August 1, 2004
Author(s)
J P. Jakupciak, Wenhua Wang, Peter E. Barker, S Srivastava, Donald H. Atha
Activation of telomerase plays a critical role in unlimited proliferation and immortalization of cells. Telomerase activity has been shown to correlate with tumor progression, indicating that tumors expressing this enzyme posses aggressive clinical

Molecular Biomarkers Used to Detect Cellular/Genetic Damage in Tissue-Engineered Skin

January 1, 2004
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, H Rodriguez
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, Tissue engineered skin (TestSkin II) was obtained, separated into its two cellular layers

Biomarkers Used to Detect Genetic Damage in Tissue-Engineered Skin

January 1, 2003
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, M. Dizdaroglu, H Rodriguez
The goal of the research was to help identify cellular biomarkers and assess measurement technologies that will help manufacturers determine how cells react to inflammatory stimuli from different polymer matrixes and assure that tissue-engineered medical

High-Throughput Analysis of Telomerase by Capillary Electrophoresis

January 1, 2003
Author(s)
Donald H. Atha, K Miller, A D. Sanow, J Xu, J L. Hess, O C. Wu, Wenhua Wang, S Srivastava, W E. Highsmith
The enzyme telomerase is expressed in 85-90% of all human cancers, but not in normal, non-stem cell somatic tissues. Clinical assays for telomerase in easily obtained body fluids would have great utility as non-invasive, cost-effective methods for the

Biomarkers of TP53 Mutation and Y-Chromosome Loss Used to Detect Cellular/Genetic Damage in Tissue Engineered Skin

September 1, 2002
Author(s)
H Rodriguez, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell
Tissue engineering is an emerging area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. Fundamentals to many tissue-engineered

Standardization of PCR Amplification for Fragile X Trinucleotide Repeat Measurements

January 1, 2002
Author(s)
C D. O'Connell, Donald H. Atha, J P. Jakupciak, Kristy L. Richie
To provide the clinical diagnostics community with accurate protocols and measurements for the detection of genetic disorders, we have established a quantitative a quantitative measurement program for trinucleotide repeats associated with human disease. In