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Displaying 976 - 1000 of 1280

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Crystal Structure of the Complex Between Thrombin and the Central "E" Region of Fibrin

March 2, 2004
Author(s)
I Pechik, J Madrazo, M W. Mosesson, I Hernandez, G L. Gilliland, L Medved
Non-substrate interactions of thrombin with fibrin play an important role in modulating its procoagulant activity. To establish the structural basis for these interactions, we crystallized PPACK-inhibited thrombin in complex with a fragment, Eht

Dynamics of Proton Transfer in Bacteriorhodopsin

February 25, 2004
Author(s)
Y. S. Lee, Morris Krauss
Proton transfer from the cyctoplasm to the extracellular side is initiated from protonated asp96 in the cytoplasmic region towards the deprotonated Schiff base. This occurs in the transition from the late M photocycle state to the N state. A quantum

Molecular Biomarkers Used to Detect Cellular/Genetic Damage in Tissue-Engineered Skin

January 1, 2004
Author(s)
C D. O'Connell, Peter E. Barker, M A. Marino, P McAndrew, Donald H. Atha, Pawel Jaruga, M Birincioglu, H Rodriguez
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, Tissue engineered skin (TestSkin II) was obtained, separated into its two cellular layers

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

Electrostatic Influence on Ion Transport through the alpha HL Channel

October 1, 2003
Author(s)
M Misakian, John J. Kasianowicz
The current-voltage relationship of a single Staphylococcus aureus α-hemolysin(αHL) channel is nonlinear, rectifying, and depends on the bulk pH and the ionic strength. The data are described qualitatively by a simple one-dimensional Nernst-Planck analysis

Fluorescence Thermometry in Microfluidics

September 29, 2003
Author(s)
David J. Ross, Laurie E. Locascio
Two techniques are described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. In the first technique, a single, strongly temperature-dependent fluorophore, rhodamine B, is used as the basis for

Phase Transitions Within the Isolated Polymer Molecule: Coupling of the Polymer Threading a Membrane Transition to the Helix-Random Coil, the Collapse, the Adsorption, and the Equilibrium Polymerization Transitions

September 22, 2003
Author(s)
Edmund A. DiMarzio, John J. Kasianowicz
The polymer threading a membrane transition (PTM), which is a first-order thermodynamic phase transition for an isolated linear polymer in the limit of infinite molecular weight, is coupled to the other 4 phase transitions of the isolated polymer molecule

Kinetic Isotope Effects in the Active Site of B-Subtilis Chorismate Mutase

September 15, 2003
Author(s)
S. E. Worthington, A. E. Roitberg, Morris Krauss
Kinetic Isotope Effects are determined for the enzyme-catalyzed Claisen rearrangement of chorismate to prephenate using computational methods. The reported kinetic isotope effects compare reasonably well with the few available experimental values. The

Cloning and Expression of the Gene for a Novel Protein From Mycobacterium Smegmatis With Functional Similarity to Eukaryotic Calmodulin

September 1, 2003
Author(s)
Prasad T. Reddy, C R. Prasad, P H. Reddy, D J. Reeder, K H. McKenney, H Jaffe, M N. Dimitrova, A Ginsburg, A Peterkofsky, P S. Murthy
The calmodulin-like protein from Mycobacterium smegmatis (MSM-CAMLP) was purified to homogeneity with a yield of about 1 g from 70 g of cells. Peptide sequence analysis of the MSM-CAMLP was used to design degenerate oligonucleotides for the eight
Displaying 976 - 1000 of 1280
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