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Displaying 776 - 800 of 1612

Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins

April 22, 2013
Author(s)
Antony K. Chen, Frank W. DelRio, Alexander W. Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L. Plant
The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevant cell

Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1

April 9, 2013
Author(s)
Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we present

Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments

April 9, 2013
Author(s)
Ross J. Haynes, Jim Huggett, Carole Foy, Vladimir Benes, Kerry Emslie, Jeremy Garson, Jan Hellemans, Mikael Kubista, Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer, Stephen Bustin
There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acid, facilitating the measurement of small

Salivary Antigen-5/CAP Family Members Are Cu 2+ -dependent Antioxidant Enzymes That Scavenge O 2 -. and Inhibit Collagen-induced Platelet Aggregation and Neutrophil Oxidative Burst

April 4, 2013
Author(s)
Teresa Assumpcao, Dongying Ma, Alexandra Schwarz, Karine Reiter, Jaime Santana, John Andersen, Jose Ribeiro, Glen Nardone, Lee L. Yu, Ivo Francischetti
The function of the antigen-5/CAP family of proteins found in the salivary gland of bloodsucking animals has remained elusive for decades. Antigen-5 members from the hematophagous insects Dipetalogaster maxima (DMAV) and Triatoma infestans (TIAV) were

Spectral Light source Distribution variations to enhance discrimination of the common bile duct from surroundings in reflectance hyperspectral imaging

March 8, 2013
Author(s)
Maritoni A. Litorja, Mira Fein, Eleanor Wehner, Karel Zuzak, Edward Livingston
The classification of anatomical features using hyperspectral imaging has been a common goal in biomedical hyperspectral imaging. Identification and location of the common bile duct is critical in cholecystectomies, one of the most common surgical

Gradient Nanofiber Scaffold Libraries for Screening Cell Response to Poly(e-caprolactone)-Calcium Phosphate Composites

March 1, 2013
Author(s)
Carl G. Simon Jr., Limin Sun, Laurence C. Chow, William Miles, Christopher K. Tison, Kaushik Chatterjee, Marian F. Young, Vinoy Thomas, Murugan Ramalingam
A 2-spinnerette approach has been developed for fabricating nanofiber scaffold gradients for use as 1) scaffold libraries for screening the effect of nanofiber properties on cell response and 2) templates for generating graded tissue interfaces. Although

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

March 1, 2013
Author(s)
Ross J. Haynes, Margaret C. Kline, Blaza Toman, Calum Scott, Paul Wallace, John M. Butler, Marcia J. Holden
Cytomegalovirus (CMV) is one of a number of human viruses that are ubiquitous in human populations, generally cause little illness upon infecting healthy individuals, but can cause life threatening disease in immune-compromised individuals. An important

Modification of tethered bilayers by phospholipid exchange with vesicles

February 27, 2013
Author(s)
David J. Vanderah, Rima Budvytyte, Mindaugas Mickevicius, Gintaras Valincius, Frank Heinrich
Phosphatidylcholine and cholesterol exchange between vesicles and planar tethered bilayer lipid membranes (tBLMs) was demonstrated from electrochemical impedance spectroscopy (EIS), fluorescence microscopy (FM), and neutron reflectometry (NR) data

Mouse Cell Line Authentication

February 13, 2013
Author(s)
Jamie L. Almeida, Carolyn R. Steffen, Kenneth D. Cole
The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed a multiplex polymerase

Thermococcus kodakarensis DNA replication

February 2, 2013
Author(s)
Zvi Kelman, Lori Kelman, Zhuo Li
DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has

Breast Cancer Biomarker Measurements and Standards

January 24, 2013
Author(s)
Kenneth D. Cole, Lili Wang, Hua-Jun He
Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result
Displaying 776 - 800 of 1612
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