Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins

Published

Author(s)

Antony K. Chen, Frank W. DelRio, Alexander W. Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L. Plant

Abstract

The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevant cell behaviors and that the mechanical properties of the fibrils can be modulated to test the effect of environmental mechanics on cells. In the present study, we engineered extracellular matrices consisting of a combination of fibrillar collagen plus fibronectin and showed that cells respond to this matrix distinctively. In particular, cells exhibit intermediate phenotypic characteristics compared to their response to collagen or fibronectin alone. These responses can be further controlled by varying fibronectin density. Cell engagement with the surface could be blocked by peptides containing the sequence Arginine-Glycine-Aspartic acid (RGD) or antibodies to α1 integrin, suggesting that cell-matrix engagement was mediated by integrin receptors that recognize both collagen and fibronectin. Regardless of the integrin engagement, cells are sensitive to the mechanical differences of the matrix. Overall, these results indicated that the fibronectin/collagen combined matrix may be a useful model system for quantifying the relative contributions of multiple ECM factors on cell responses and the underlying signaling pathways.
Citation
Biomaterials

Keywords

ECM, Fibronectin, Collagen, Stiffness

Citation

Chen, A. , DelRio, F. , Peterson, A. , Chung, K. , Bhadriraju, K. and Plant, A. (2013), Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins, Biomaterials, [online], https://doi.org/10.1002/bit.24921 (Accessed June 19, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created April 21, 2013, Updated October 12, 2021